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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • p53 Antibodies

          Invitrogen

          p53 Monoclonal Antibody (DO-7)

          Advanced Verification
          21 Published Figures
          174 References
          View all (364) p53 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite p53 Monoclonal Antibody (DO-7)

          • Antibody Testing Data (15)
          • Published Figures (21)
          • Advanced Verification (7)
          p53 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          p53 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 43

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          p53 Antibody (MA5-12557) in ICC/IF

          Immunofluorescence analysis of Cellular tumor antigen p53 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with p53 Monoclonal Antibody (DO-7) (Product # MA5-12557) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          p53 Antibody in Immunocytochemistry (ICC/IF)
          p53 Antibody in Immunocytochemistry (ICC/IF)
          p53 Antibody in Immunocytochemistry (ICC/IF)
          p53 Antibody in Immunocytochemistry (ICC/IF)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Flow Cytometry (Flow)
          p53 Antibody in ChIP Assay (ChIP)
          p53 Antibody in ChIP Assay (ChIP)
          p53 Antibody in Immunohistochemistry (IHC)
          p53 Antibody in Immunohistochemistry (IHC)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Immunocytochemistry (ICC/IF)
          p53 Antibody in Immunocytochemistry (ICC/IF)
          p53 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)
          p53 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)
          p53 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          p53 Antibody in Immunohistochemistry (IHC)
          p53 Antibody in Immunohistochemistry (IHC)
          p53 Antibody in Immunohistochemistry (IHC)
          p53 Antibody in Immunohistochemistry (IHC)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          p53 Antibody in Western Blot (WB)
          p53 Antibody
          p53 Antibody
          p53 Antibody
          p53 Antibody
          p53 Antibody
          p53 Antibody
          p53 Antibody

          Product Details

          MA5-12557

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:4,000
          View 49 publications 49 publications

          Immunohistochemistry (IHC)

          -
          View 102 publications 102 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:200
          View 5 publications 5 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 8 publications 8 publications

          Flow Cytometry (Flow)

          1:50
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          1:50-1:100
          View 3 publications 3 publications

          ChIP assay (ChIP)

          1 µg/mL
          -

          Miscellaneous PubMed (MISC)

          -
          View 5 publications 5 publications
          Product Specifications

          Species Reactivity

          Bovine, Human

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2b, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          DO-7

          Immunogen

          Recombinant human wild-type p53 protein expressed in E. coli

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.05 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10989883

          Product Specific Information

          MA5-12557 targets p53 in FACS, ICC/IF, IHC (P), IP, ChIP, and WB applications and shows reactivity with Human and bovine samples. This antibody is not suitable for mouse colon tissue in IHC applications.

          The MA5-12557 immunogen is recombinant human wild-type p53 protein expressed in E. coli.

          This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

          Target Information

          The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant tumor protein 53; OTTMUSP00000006194; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; Phosphoprotein p53; transformation-related protein 53; tumor protein 53; tumor protein p53 (Li-Fraumeni syndrome); Tumor suppressor p53; tumor supressor p53; Tumour Protein p53

          View more View less

          Gene Aliases: BCC7; BOS_18908; LFS1; P53; TP53; TRP53

          View more View less

          UniProt ID: (Bovine) P67939, (Human) P04637

          View more View less

          Entrez Gene ID: (Bovine) 281542, (Human) 7157

          View more View less

          Function(s)
          transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding protease binding p53 binding DNA binding chromatin binding damaged DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding copper ion binding ATP binding protein kinase binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone deacetylase regulator activity histone acetyltransferase binding identical protein binding sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding MDM2/MDM4 family protein binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein binding transcription factor binding zinc ion binding enzyme binding protein phosphatase binding protein self-association
          Process(es)
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter DNA strand renaturation in utero embryonic development somitogenesis release of cytochrome c from mitochondria T cell proliferation involved in immune response B cell lineage commitment T cell lineage commitment response to ischemia nucleotide-excision repair double-strand break repair apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator ER overload response transforming growth factor beta receptor signaling pathway Ras protein signal transduction multicellular organism development gastrulation negative regulation of neuroblast proliferation central nervous system development cell aging negative regulation of DNA replication determination of adult lifespan rRNA transcription response to salt stress response to X-ray response to gamma radiation positive regulation of cardiac muscle cell apoptotic process negative regulation of cell growth negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of histone deacetylation mitotic G1 DNA damage checkpoint positive regulation of protein oligomerization T cell differentiation in thymus regulation of tissue remodeling cellular response to UV multicellular organism growth cellular response to drug cellular response to glucose starvation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of apoptotic process positive regulation of neuron apoptotic process response to antibiotic regulation of mitochondrial membrane permeability negative regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation protein tetramerization chromosome organization neuron apoptotic process positive regulation of thymocyte apoptotic process cellular response to hypoxia cellular response to ionizing radiation mitotic cell cycle arrest positive regulation of release of cytochrome c from mitochondria positive regulation of cell aging replicative senescence oxidative stress-induced premature senescence oligodendrocyte apoptotic process negative regulation of macromitophagy negative regulation of reactive oxygen species metabolic process positive regulation of reactive oxygen species metabolic process positive regulation of intrinsic apoptotic signaling pathway base-excision repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein complex assembly cellular response to DNA damage stimulus cell cycle arrest protein localization cell proliferation negative regulation of cell proliferation positive regulation of gene expression viral process protein sumoylation cell differentiation DNA damage response, signal transduction by p53 class mediator chromatin assembly regulation of apoptotic process positive regulation of apoptotic process entrainment of circadian clock by photoperiod proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of protein export from nucleus circadian behavior negative regulation of helicase activity negative regulation of telomerase activity positive regulation of cell cycle arrest intrinsic apoptotic signaling pathway by p53 class mediator intrinsic apoptotic signaling pathway positive regulation of execution phase of apoptosis positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway regulation of signal transduction by p53 class mediator regulation of cell cycle G2/M phase transition positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
          It has to be done as per old AB suggested Products section.
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