Alomone Labs, LTD

pan ASIC (extracellular) Polyclonal Antibody

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

ASC-031-200UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Frozen) (IHC (F))

1:400
-

Immunocytochemistry (ICC/IF)

1:25
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)TRYGKELSMVKIPSK, corresponding to amino acid residues 368-382 of rat ASIC1, Extracellular loop
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ASIC1 is permeable for Ca(2+), Li(+) and K(+) and is expressed in dorsal root ganglia, sciatic nerves, olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. It is diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acid sensing (proton gated) ion channel 1; acid sensing (proton gated) ion channel 2; acid sensing (proton gated) ion channel 3; acid sensing (proton gated) ion channel family member 4; acid sensing ion channel; acid sensing ion channel 1; acid sensing ion channel 2; acid sensing ion channel 3; acid sensing ion channel family member 4; acid-sensing (proton-gated) ion channel 1; acid-sensing (proton-gated) ion channel 2; acid-sensing (proton-gated) ion channel 3; acid-sensing (proton-gated) ion channel family member 4; Acid-sensing ion channel; Acid-sensing ion channel 1; acid-sensing ion channel 1a protein; Acid-sensing ion channel 2; Acid-sensing ion channel 3; Acid-sensing ion channel 4; Amiloride-sensitive brain sodium channel; Amiloride-sensitive brain sodium channel 2; Amiloride-sensitive cation channel 1, neuronal; amiloride-sensitive cation channel 1, neuronal (degenerin); Amiloride-sensitive cation channel 2, neuronal; Amiloride-sensitive cation channel 3; amiloride-sensitive cation channel 3, testis; Amiloride-sensitive cation channel 4; Amiloride-sensitive cation channel 4, pituitary; amiloride-sensitive cation channel family member 4, pituitary; Amiloride-sensitive cation channel neuronal 1; ASIC1; ASIC2; ASIC3; ASIC4; BNaC2; BNC1; Brain sodium channel 1; Brain sodium channel 2; brain sodium channel 4; Cation channel, amiloride-sensitive, neuronal, 2; degenerin; degenerin 2; Dorsal root ASIC; DRASIC; hTNaC1; Mammalian degenerin homolog; MDEG; modulatory subunit of ASIC2a; neuronal amiloride-sensitive cation channel 1; Neuronal amiloride-sensitive cation channel 3; proton gated cation channel ASIC1; proton gated cation channel DRASIC; proton-gated cation channel subunit; putative acid-sensing ion channel; spinal chord ASIC; Spinal cord ASIC; Testis sodium channel 1

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Gene Aliases: ACCN; ACCN1; ACCN2; ACCN3; ACCN4; ACIC2; AI843610; ASIC; ASIC1; ASIC1a; ASIC2; ASIC2a; ASIC3; ASIC4; AW742291; B530003N02Rik; BNAC1; BNaC1a; BNAC2; BNAC4; BNC1; BNC1k; DRASIC; hBNaC1; MDEG; MDEG1; MDEG2; SLNAC1; Spasic; TNAC1

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UniProt ID: (Human) P78348, (Human) Q16515, (Human) Q9UHC3, (Human) Q96FT7, (Mouse) Q6NXK8, (Rat) P55926, (Rat) Q62962, (Mouse) Q925H0, (Mouse) Q6X1Y6, (Rat) O35240, (Rat) Q9JHS6, (Mouse) Q7TNS7

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Entrez Gene ID: (Human) 41, (Human) 40, (Human) 9311, (Human) 55515, (Mouse) 11419, (Rat) 79123, (Rat) 25364, (Mouse) 11418, (Mouse) 171209, (Rat) 286920, (Rat) 63882, (Mouse) 241118

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Function(s)
protein binding ligand-gated sodium channel activity ion gated channel activity acid-sensing ion channel activity cation channel activity sodium channel activity PDZ domain binding enterobactin transporter activity ion channel activity sodium ion transmembrane transporter activity monovalent inorganic cation transmembrane transporter activity voltage-gated sodium channel activity
Process(es)
transport ion transport cation transport sodium ion transport response to heat response to mechanical stimulus response to acidic pH ion transmembrane transport sodium ion transmembrane transport enterobactin transport detection of chemical stimulus involved in sensory perception sensory perception of sour taste detection of temperature stimulus involved in sensory perception detection of temperature stimulus involved in sensory perception of pain detection of mechanical stimulus involved in sensory perception of pain detection of chemical stimulus involved in sensory perception of pain detection of mechanical stimulus involved in sensory perception cation transmembrane transport calcium ion transport memory associative learning monovalent inorganic cation transport regulation of membrane potential negative regulation of neurotransmitter secretion calcium ion transmembrane transport cellular response to pH sensory perception of pain protein homotrimerization regulation of systemic arterial blood pressure by aortic arch baroreceptor feedback phototransduction sensory perception of sound regulation of gene expression regulation of vasoconstriction regulation of blood coagulation regulation of ion transmembrane transport protein localization to synapse cellular response to drug negative regulation of apoptotic process positive regulation of synapse assembly cellular response to acidic pH positive regulation of cation channel activity chemical synaptic transmission central nervous system development peripheral nervous system development signal transduction response to pH sensory perception
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