Bioss

pan Cytokeratin Polyclonal Antibody

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (8)

pan Cytokeratin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

pan Cytokeratin Antibody (BS-1712R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:400 overnight at 4°C, DAB staining.

pan Cytokeratin Antibody in Western Blot (WB)

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Product Details

BS-1712R

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:100-1:500

Flow Cytometry (Flow)

1:20-1:100

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human pan cytokeratin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes. In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 57kd keratin; 57kDa keratin; 58 kDa cytokeratin; 67 kDa cytokeratin; caracul; CK 2e; CK-1; CK-2e; CK-4; CK-5; CK-6G; CK-7; CK-71; CK-72; CK-75; CK-78; CK-8; cytokeratin 1; cytokeratin 4; cytokeratin 5; Cytokeratin 7; cytokeratin 8; Cytokeratin endo A; cytokeratin KRT2-6HF; cytokeratin type II; Cytokeratin-1; Cytokeratin-2e; Cytokeratin-4; Cytokeratin-5; Cytokeratin-6G; Cytokeratin-7; Cytokeratin-71; Cytokeratin-72; Cytokeratin-75; Cytokeratin-78; Cytokeratin-8; Cytoskeletal 57 kDa keratin; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; Epithelial keratin-2e; Hair alpha protein; hK6hf; hK6irs; hK6irs1; K1; K2e; K4; K5; K6G; K7; K71; K72; K75; K78; K8; keratin 1, type II; keratin 2 (epidermal ichthyosis bullosa of Siemens); keratin 2 epidermis; keratin 2, type II; keratin 2A; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; Keratin 6 irs; keratin 6, inner root sheath, 2; keratin 7, type II; keratin 71; keratin 71, type II; keratin 72, pseudogene; keratin 72, type II; keratin 75, type II; keratin 78, type II; keratin 8, type II; keratin 86; keratin complex 2, basic, gene 1; keratin complex 2, basic, gene 17; keratin complex 2, basic, gene 35; keratin complex 2, basic, gene 4; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 6g; keratin complex 2, basic, gene 7; keratin complex 2, basic, gene 8; keratin complex 2, gene 17; keratin complex 2, gene 6g; keratin Kb40; keratin protein K6irs; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 2 epidermal; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 7; Keratin, type II cytoskeletal 71; Keratin, type II cytoskeletal 72; Keratin, type II cytoskeletal 75; Keratin, type II cytoskeletal 78; Keratin, type II cytoskeletal 8; Keratin-1; Keratin-2 epidermis; Keratin-2e; Keratin-4; Keratin-5; Keratin-5b; Keratin-6 hair follicle; Keratin-6G; Keratin-7; Keratin-71; Keratin-72; Keratin-75; Keratin-78; Keratin-8; keratinocyte associated protein 1; krt1 {ECO:0000250|UniProtKB:P04264}; krt2; krt2 {ECO:0000250|UniProtKB:P35908}; krt4 {ECO:0000250|UniProtKB:P19013}; krt5; krt7; krt7 {ECO:0000250|UniProtKB:P08729}; mK6hf; mK6irs; mK6irs1/Krt2-6g; Sarcolectin; tuftelin interacting protein 8; Type II inner root sheath-specific keratin-K6irs1; Type II inner root sheath-specific keratin-K6irs2; type II keratin Kb1; type II keratin Kb18; type II keratin Kb2; type II keratin Kb35; type II keratin Kb4; type II keratin Kb40; type II keratin Kb7; type II keratin-18; Type II keratin-K6hf; type II mesothelial keratin K7; Type-II keratin Kb1; Type-II keratin Kb18; Type-II keratin Kb2; Type-II keratin Kb34; Type-II keratin Kb35; Type-II keratin Kb4; Type-II keratin Kb40; Type-II keratin Kb5; Type-II keratin Kb7; Type-II keratin Kb8; type-II keratin-35

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Gene Aliases: 2310030B04Rik; 3300001P10Rik; 4732468K03Rik; AA589387; AA589543; AA960620; AI507495; AL022697; AU019895; AW108092; AW146334; BB005427; Ca; Cal4; CARD2; CK-2e; CK-4; CK-78; CK-8; CK1; CK4; CK5; CK7; CK8; Cu; CYK4; CYK8; CYKER; D15Wsu77e; DDD; DDD1; EBS2; EHK; EHK1; EndoA; EPPK; HYPT13; K1; K2C7; K2C8; K2e; K4; K5; K5B; K6HF; K6irs; K6IRS1; K6IRS2; K7; K78; K8; Kb1; KB18; Kb2; KB34; KB35; Kb4; KB40; Kb7; KO; Krt-2.1; Krt-2.4; Krt-2.8; KRT1; KRT1A; KRT2; Krt2-1; Krt2-17; Krt2-2; Krt2-35; Krt2-4; Krt2-5; Krt2-6g; Krt2-6hf; Krt2-7; Krt2-8; KRT2A; KRT2E; KRT4; KRT5; KRT5A; KRT6; Krt6g; KRT6IRS; KRT6IRS1; KRT6IRS2; KRT7; KRT71; KRT72; Krt72-ps; KRT75; KRT78; KRT8; Krt86; KRTA; Krtcap1; KRTE; mK6irs; mK6irs1; NEPPK; PFB; Sak57; SCL; Tfip8; WSN1