OriGene

pan Cytokeratin (pan CK) Monoclonal Antibody (AE1+AE3), TrueMAB™

pan Cytokeratin (pan CK) Antibody in Immunocytochemistry (ICC/IF)

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pan Cytokeratin (pan CK) Antibody (CF190321) in ICC/IF

Immunofluorescence analysis of pan Cytokeratin was performed using 70% confluent log phase A-431 cells. The cells were fixed and permeabilized with ice-cold acetone at 4°C for 5 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with pan Cytokeratin (pan CK) Monoclonal Antibody (AE1+AE3), TrueMAB™ (Product # CF190321, 5 µg/mL) in 0.1% BSA, incubated at 4 degree celsius ov... View More

pan Cytokeratin (pan CK) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

pan Cytokeratin (pan CK) Antibody in Western Blot (WB)

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Product Details

CF190321

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:150

Immunocytochemistry (ICC/IF)

5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AE1+AE3

Immunogen

Human epidermal cytokeratins

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 39.1; 40-kDa keratin intermediate filament; 47 kDa cytokeratin; 56 kDa cytokeratin; 57kd keratin; 57kDa keratin; 58 kDa cytokeratin; 60-kDa keratin; 65 kDa cytokeratin; 67 kDa cytokeratin; Cell proliferation-inducing gene 46 protein; CK-1; CK-10; CK-13; CK-14; CK-15; CK-16; CK-17; CK-18; CK-19; CK-1B; CK-2e; CK-2P; CK-3; CK-4; CK-5; CK-6A; CK-6D; CK-7; CK-8; cytokeratin 1; cytokeratin 10; cytokeratin 13; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 2; cytokeratin 3; cytokeratin 4; cytokeratin 5; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin 7; cytokeratin 8; Cytokeratin endo A; Cytokeratin endo B; Cytokeratin-1; Cytokeratin-10; Cytokeratin-13; Cytokeratin-14; Cytokeratin-15; Cytokeratin-16; Cytokeratin-17; Cytokeratin-18; Cytokeratin-19; Cytokeratin-1B; Cytokeratin-2e; Cytokeratin-2P; Cytokeratin-3; Cytokeratin-4; Cytokeratin-5; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; Cytokeratin-7; Cytokeratin-8; Cytoskeletal 57 kDa keratin; embryonic type II keratin 1; Embryonic type II keratin-1; Endo B; epidermal keratin 10; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolysis bullosa simplex, Dowling-Meara, Koebner; epidermolytic hyperkeratosis 1; Epithelial keratin-2e; focal non-epidermolytic palmoplantar keratoderma; Hair alpha protein; HMWCK; intermediate filament protein; K1; K10; K13; K14; K15; K16; K17; K18; K19; K1B; K2e; K2P; K3; K4; K5; K6A; K7; K76; K77; K8; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 1, type II; keratin 10, type I; keratin 13, type I; keratin 14, type I; keratin 15, type I; keratin 16, type I; keratin 17, type I; keratin 18, type I; keratin 19, type I; Keratin 1B; keratin 2 epidermis; keratin 2, type II; keratin 2p; keratin 3, type II; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6A, , type II; keratin 6A, type II; keratin 7, type II; keratin 76, type II; keratin 77, type II; keratin 8, type II; keratin 86; keratin complex 1, acidic, gene 10; keratin complex 1, acidic, gene 13; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 15; keratin complex 1, acidic, gene 16; keratin complex 1, acidic, gene 17; keratin complex 1, acidic, gene 18; keratin complex 1, acidic, gene 19; keratin complex 2, basic, gene 1; keratin complex 2, basic, gene 17; keratin complex 2, basic, gene 39; keratin complex 2, basic, gene 4; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin complex 2, basic, gene 7; keratin complex 2, basic, gene 8; keratin complex 2, gene 17; keratin complex 2, gene 6a; Keratin D; keratin intermediate filament 16a; keratin intermediate filament 16b; keratin K6h; Keratin type II cytoskeletal 5; keratin, 55K type II cytoskeletal; keratin, epidermal type II, K6A; keratin, simple epithelial type I, K7; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 13; Keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; Keratin, type I cytoskeletal 17; Keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; Keratin, type I cytoskeletal 59 kDa; keratin, type I, 40-kd; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 1b; Keratin, type II cytoskeletal 2 epidermal; Keratin, type II cytoskeletal 2 oral; Keratin, type II cytoskeletal 3; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 7; Keratin, type II cytoskeletal 8; Keratin-1; Keratin-10; Keratin-13; Keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; Keratin-16; Keratin-17; Keratin-18; Keratin-19; Keratin-2 epidermis; Keratin-2e; Keratin-3; Keratin-4; Keratin-5; Keratin-6-alpha; Keratin-6A; keratin-6C; Keratin-7; Keratin-76; Keratin-77; Keratin-8; KRT1B; krt2; krt5; krt7; krt76; mK6-alpha; pan Cytokeratin; pan keratin; pankeratin; Sarcolectin; suprabasal cytokeratin 10; tuftelin interacting protein 8; type I cytoskeletal 15; type II cytoskeletal 1b; type II keratin Kb39; type II mesothelial keratin K7; type-II intermediate filament protein; Type-II keratin Kb1; type-II keratin Kb12; Type-II keratin Kb2; Type-II keratin Kb3; Type-II Keratin Kb39; Type-II keratin Kb4; Type-II keratin Kb5; Type-II keratin Kb6; Type-II keratin Kb7; Type-II keratin Kb8; Type-II keratin Kb9

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Gene Aliases: 2310001L23Rik; 2310016L08Rik; 3300001P10Rik; 39.1; 4732484G22Rik; AA960620; AI324768; AI528832; AI626930; AI663979; AL022697; AU019895; AW108092; AW146334; BB005427; BCIE; BIE; CARD2; CK-17; CK-18; CK-2e; CK-4; CK-6C; CK-6E; CK-8; CK1; CK10; CK13; CK14; CK15; CK16; CK18; CK19; CK3; CK4; CK5; CK6A; CK6C; CK6D; CK7; CK8; CYK18; CYK4; CYK8; D130054E02Rik; D15Wsu77e; DDD; DDD1; EBS2; EBS3; EBS4; EHK; EHK1; EndoA; EndoC; EPPK; FNEPPK; HUMCYT2A; K1; K10; K13; K14; K15; K16; K17; K18; K19; K1B; K1C1; K1CO; K1CP; K1CS; K1emb; K2C7; K2C8; K2e; K3; K4; K5; K6A; K6C; K6D; K7; K8; Ker2; Kerd; KO; KPP; Krt-1.10; Krt-1.13; Krt-1.14; Krt-1.19; Krt-2.1; Krt-2.4; Krt-2.8; KRT1; Krt1-10; Krt1-13; Krt1-14; Krt1-15; Krt1-16; Krt1-17; Krt1-18; Krt1-19; KRT10; KRT13; KRT14; KRT15; KRT16; KRT16A; KRT17; KRT18; KRT19; KRT1A; KRT1B; KRT2; Krt2-1; Krt2-17; Krt2-2; Krt2-4; Krt2-5; Krt2-6; Krt2-6a; Krt2-6c; Krt2-7; Krt2-8; Krt239; KRT2A; KRT2B; KRT2E; KRT2P; KRT3; KRT4; KRT5; KRT5A; Krt6; KRT6A; KRT6C; KRT6D; KRT7; KRT76; KRT77; KRT8; Krt86; KRTA; KRTB; KRTE; mK1b; mK6[a]; MK6a; NEPPK; NFJ; PC; PC1; PC2; PC3; PCHC1; PIG46; SCL; Tfip8; WSN1; WSN2