Proteintech

tPA Polyclonal Antibody

tPA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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tPA Antibody (10147-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue slide using (Product # 10147-1-AP) tPA antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

tPA Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

tPA Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10147-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

tPA Fusion Protein Ag0200 (356-516 aa encoded by BC002795)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TYDNDIALL QLKSDSSRCA QESSVVRTVC LPPADLQLPD WTECELSGYG KHEALSPFYS ERLKEAHVRL YPSSRCTSQH LLNRTVTDNM LCAGDTRSGG PQANLHDACQ GDSGGPLVCL NDGRMTLVGI ISWGLGCGQK DVPGVYTKVT NYLDWIRDNM RP (356-516 aa encoded by BC002795)

Target Information

TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physio pathological events. It specifically cleaves the Arg- -Val bond in plasminogen to form plasmin, and is comprised of a heterodimer of chain A and chain B held by a disulfide bond. TPA binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Novel silk protein/hyaluronic acid hydrogel loaded with azithromycin as an immunomodulatory barrier to prevent postoperative adhesions.

International journal of biological macromolecules

Tian L,Sun T,Fan M,Lu H,Sun C

Sun Apr 30 00:00:00 UTC 2023

Clostridium butyricum RH2 Alleviates Chronic Foot Shock Stress-Induced Behavioral Deficits in Rats via PAI-1.

Frontiers in pharmacology

Zhang W,Ding T,Zhang H,Chen Y,Liu L,Jiang J,Song S,Cheng H,Wu C,Sun J,Wu Q

Sat Jul 16 00:00:00 UTC 2022

Clostridium butyricum RH2 Alleviates Chronic Foot Shock Stress-Induced Behavioral Deficits in Rats via PAI-1.

Frontiers in pharmacology

Zhang W,Ding T,Zhang H,Chen Y,Liu L,Jiang J,Song S,Cheng H,Wu C,Sun J,Wu Q

Sat Jul 16 00:00:00 UTC 2022

FUNDC1-dependent mitophagy induced by tPA protects neurons against cerebral ischemia-reperfusion injury.

Redox biology

Cai Y,Yang E,Yao X,Zhang X,Wang Q,Wang Y,Liu J,Fan W,Yi K,Kang C,Wu J

Fri Jan 01 00:00:00 UTC 2021

FUNDC1-dependent mitophagy induced by tPA protects neurons against cerebral ischemia-reperfusion injury.

Redox biology

Cai Y,Yang E,Yao X,Zhang X,Wang Q,Wang Y,Liu J,Fan W,Yi K,Kang C,Wu J

Fri Jan 01 00:00:00 UTC 2021

Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway.

Neuropsychiatric disease and treatment

Dong H,Qin YQ,Sun YC,Yao HJ,Cheng XK,Yu Y,Lu SS

Fri Apr 22 00:00:00 UTC 2022

Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway.

Neuropsychiatric disease and treatment

Dong H,Qin YQ,Sun YC,Yao HJ,Cheng XK,Yu Y,Lu SS

Fri Apr 22 00:00:00 UTC 2022

2,5-Hexanedione induced apoptosis in rat spinal cord neurons and VSC4.1 cells via the proNGF/p75NTR and JNK pathways.

Bioscience reports

Luo M,Shi X,Guo Q,Li S,Zhang Q,Sun X,Piao F

Fri Apr 30 00:00:00 UTC 2021

Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer.

Frontiers in oncology

Tang H,Zheng J,Bai X,Yue KL,Liang JH,Li DY,Wang LP,Wang JL,Guo Q

Sat Mar 30 00:00:00 UTC 2024

Sevoflurane leads to learning and memory dysfunction via breaking the balance of tPA/PAI-1.

Neurochemistry international

Dong Y,Hong W,Tang Z,Gao Y,Wu X,Liu H

Thu Oct 01 00:00:00 UTC 2020

The Intestinal Microbiota Plays as a Protective Regulator Against Radiation Pneumonitis.

Radiation research

Nie X,Li L,Yi M,Qin W,Zhao W,Li F,Wu B,Yuan X

Wed Jul 08 00:00:00 UTC 2020

NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.

Aging

Ma P,Li G,Jiang X,Shen X,Li H,Yang L,Liu W

Sat Dec 19 00:00:00 UTC 2020

Lipoprotein receptor loss in forebrain radial glia results in neurological deficits and severe seizures.

Glia

Bres EE,Safina D,Müller J,Bedner P,Yang H,Helluy X,Shchyglo O,Jansen S,Mark MD,Esser A,Steinhäuser C,Herlitze S,Pietrzik CU,Sirko S,Manahan-Vaughan D,Faissner A

Tue Dec 01 00:00:00 UTC 2020

Bottom up proteomics reveals novel differentiation proteins in neuroblastoma cells treated with 13-cis retinoic acid.

Journal of proteomics

Halakos EG,Connell AJ,Glazewski L,Wei S,Mason RW

Wed Oct 30 00:00:00 UTC 2019

tPA promotes the proliferation of lung fibroblasts and activates the Wnt/β-catenin signaling pathway in idiopathic pulmonary fibrosis.

Cell cycle (Georgetown, Tex.)

Chen L,Hou J,Fu X,Chen X,Wu J,Han X

Fri Nov 01 00:00:00 UTC 2019

Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells.

International journal of biological macromolecules

Sun D,Liu Y,Wang H,Deng F,Zhang Y,Zhao S,Ma X,Wu H,Sun G

Sun Apr 01 00:00:00 UTC 2018

Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells.

International journal of biological macromolecules

Sun D,Liu Y,Wang H,Deng F,Zhang Y,Zhao S,Ma X,Wu H,Sun G

Sun Apr 01 00:00:00 UTC 2018

Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells.

Scientific reports

Liu Y,Wang S,Sun D,Liu Y,Liu Y,Wang Y,Liu C,Wu H,Lv Y,Ren Y,Guo X,Sun G,Ma X

Tue Jul 19 00:00:00 UTC 2016

Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells.

Scientific reports

Liu Y,Wang S,Sun D,Liu Y,Liu Y,Wang Y,Liu C,Wu H,Lv Y,Ren Y,Guo X,Sun G,Ma X

Tue Jul 19 00:00:00 UTC 2016

Tissue plasminogen activator contributes to alterations of neuronal migration and activity-dependent responses in fragile X mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Achuta VS,Rezov V,Uutela M,Louhivuori V,Louhivuori L,Castrén ML

Wed Jan 29 00:00:00 UTC 2014

Tissue plasminogen activator contributes to alterations of neuronal migration and activity-dependent responses in fragile X mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Achuta VS,Rezov V,Uutela M,Louhivuori V,Louhivuori L,Castrén ML

Wed Jan 29 00:00:00 UTC 2014

Tryptase promotes atherosclerotic plaque haemorrhage in ApoE-/- mice.

PloS one

Zhi X,Xu C,Zhang H,Tian D,Li X,Ning Y,Yin L

Tue Oct 22 00:00:00 UTC 2013

Tryptase promotes atherosclerotic plaque haemorrhage in ApoE-/- mice.

PloS one

Zhi X,Xu C,Zhang H,Tian D,Li X,Ning Y,Yin L

Tue Oct 22 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: 3.4.21.68; alteplase; plasminogen/activator kringle; reteplase; t plasminogen activator; t-PA; t-plasminogen activator; tissue plasminogen activator; Tissue-type plasminogen activator

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Gene Aliases: AU020998; AW212668; D8Ertd2e; PATISS; PLAT; T-PA; TPA

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UniProt ID: (Human) P00750, (Mouse) P11214, (Rat) P19637

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Entrez Gene ID: (Human) 5327, (Mouse) 18791, (Rat) 25692

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Function(s)

glycoprotein binding serine-type endopeptidase activity receptor binding protein binding phosphoprotein binding peptidase activity serine-type peptidase activity hydrolase activity molecular_function

Process(es)

response to hypoxia cellular protein modification process proteolysis blood coagulation smooth muscle cell migration plasminogen activation fibrinolysis negative regulation of proteolysis platelet-derived growth factor receptor signaling pathway synaptic transmission, glutamatergic positive regulation of ovulation wound healing response to peptide hormone regulation of synaptic plasticity response to cAMP cellular response to dexamethasone stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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tPA Polyclonal Antibody

tPA Antibody (10147-1-AP) in IHC (P)

Product Details

10147-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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