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          • Primary Antibodies ›
          • uNOS Antibodies

          Invitrogen

          uNOS Monoclonal Antibody (NOS-3F7-B11 B5)

          2 Published Figures
          10 References
          View all (3) uNOS antibodies

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          Cite uNOS Monoclonal Antibody (NOS-3F7-B11 B5)

          • Antibody Testing Data (5)
          • Published Figures (2)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          uNOS Antibody (MA3-030) in ICC/IF

          Immunofluorescent analysis of uNOS (green) in SK-N-MC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody (NOS-3F7-B11 B5) (Product # MA3-030) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was st... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Western Blot (WB)
          uNOS Antibody in Western Blot (WB)
          uNOS Antibody in Western Blot (WB)

          Product Details

          MA3-030

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:50-1:500
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 8 publications 8 publications

          Immunohistochemistry (Frozen) (IHC (F))

          1:100
          -

          Immunocytochemistry (ICC/IF)

          1:20
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Bovine, Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgM

          Class

          Monoclonal

          Type

          Antibody

          Clone

          NOS-3F7-B11 B5

          Immunogen

          Purified bovine bNOS.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          ascites

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_325476

          Product Specific Information

          MA3-030 detects brain nitric oxide synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS) in bovine, human, mouse and rat tissues.

          MA3-030 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and immunohistochemical experiments. By Western blot, this antibody detects an ~130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an ~155 kDa band in tissues expressing bNOS and an ~140 kDa band in tissues expressing eNOS. Immunohistochemical staining of NOS with MA3-030 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.

          The MA3-030 antigen is purified bovine bNOS.

          Target Information

          NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: bNOS; cNOS; Constitutive NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; HEP-NOS; Hepatocyte NOS; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, brain; Nitric oxide synthase, endothelial; Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nNOS; NOS type I; NOS type II; NOS type III; NOS, type II; NOSII; NOSIII; Peptidyl-cysteine S-nitrosylase NOS1; Peptidyl-cysteine S-nitrosylase NOS2

          View more View less

          Gene Aliases: 2310005C01Rik; 2310065A03Rik; Bnos; BOS_18469; BOS_4740; ECNOS; eNOS; HEP-NOS; i-NOS; IHPS1; INOS; Inosl; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; Nos-2; Nos-3; NOS-I; NOS-II; NOS1; NOS2; NOS2A; NOS3

          View more View less

          UniProt ID: (Human) P29475, (Human) P35228, (Bovine) Q27995, (Human) P29474, (Rat) P29476, (Mouse) Q9Z0J4, (Mouse) P29477, (Rat) Q06518, (Mouse) P70313, (Rat) Q62600

          View more View less

          Entrez Gene ID: (Human) 4842, (Human) 4843, (Bovine) 282876, (Bovine) 287024, (Human) 4846, (Rat) 24598, (Mouse) 18125, (Mouse) 18126, (Rat) 24599, (Mouse) 18127, (Rat) 24600

          View more View less

          Function(s)
          nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding sodium channel regulator activity enzyme binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity ion channel binding cadmium ion binding flavin adenine dinucleotide binding NADP binding scaffold protein binding receptor binding metal ion binding actin monomer binding NADPH-hemoprotein reductase activity amino acid binding ATPase binding actin binding beta-catenin binding cAMP-dependent protein kinase regulator activity oxidoreductase activity protein kinase binding cadherin binding nitric-oxide synthase binding Hsp90 protein binding
          Process(es)
          arginine catabolic process nitric oxide biosynthetic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity negative regulation of extrinsic apoptotic signaling pathway via death domain receptors angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of sodium ion transport regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin nitric oxide mediated signal transduction regulation of blood pressure negative regulation of cell proliferation response to heat negative regulation of platelet activation negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals lung development lipopolysaccharide-mediated signaling pathway response to fluid shear stress negative regulation of potassium ion transport endothelial cell migration cell redox homeostasis positive regulation of angiogenesis negative regulation of blood pressure positive regulation of vasodilation regulation of blood vessel size regulation of nitric-oxide synthase activity negative regulation of hydrolase activity negative regulation of calcium ion transport oxidation-reduction process response to hypoxia positive regulation of leukocyte mediated cytotoxicity innate immune response in mucosa superoxide metabolic process circadian rhythm response to bacterium negative regulation of gene expression peptidyl-cysteine S-nitrosylation prostaglandin secretion cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium regulation of cellular respiration regulation of insulin secretion defense response to Gram-negative bacterium positive regulation of killing of cells of other organism cellular response to lipopolysaccharide cellular response to interferon-gamma interleukin-6 secretion interleukin-8 secretion regulation of cytokine production involved in inflammatory response inflammatory response response to lipopolysaccharide regulation of protein kinase activity signal transduction response to estradiol positive regulation of apoptotic process negative regulation of smooth muscle cell proliferation cellular response to mechanical stimulus cellular response to transforming growth factor beta stimulus muscle contraction striated muscle contraction female pregnancy aging regulation of heart contraction response to organonitrogen compound response to lead ion response to organic cyclic compound response to activity response to nutrient levels negative regulation of peptidyl-serine phosphorylation response to vitamin E multicellular organismal response to stress positive regulation of histone acetylation response to nicotine exogenous drug catabolic process negative regulation of apoptotic process response to peptide hormone response to estrogen response to ethanol negative regulation of heart contraction positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake regulation of sensory perception of pain cellular response to growth factor stimulus response to nitric oxide cellular response to epinephrine stimulus positive regulation of the force of heart contraction positive regulation of long-term synaptic potentiation positive regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of neuron death positive regulation of sodium ion transmembrane transport myoblast fusion negative regulation of calcium ion transport into cytosol neurotransmitter biosynthetic process regulation of neurogenesis regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity negative regulation of adrenergic receptor signaling pathway involved in heart process regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of cardiac conduction response to mechanical stimulus response to fructose response to metal ion response to cytokine cellular response to heat response to statin response to drug response to amino acid response to axon injury response to corticosterone response to hyperoxia response to lipoprotein particle retina development in camera-type eye bone development cellular response to magnesium ion cellular response to cAMP cellular response to tumor necrosis factor cellular response to organic cyclic compound response to candesartan response to peptide response to ketone response to salt endothelial cell proliferation G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger response to hormone glycoside metabolic process cGMP-mediated signaling response to tumor necrosis factor intracellular signal transduction response to immobilization stress wound healing retinoic acid receptor signaling pathway cellular response to lithium ion cellular response to vitamin D cellular response to interleukin-1 cellular response to redox state response to dexamethasone
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