Proteintech

uPA Polyclonal Antibody

uPA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

uPA Antibody (17968-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 17968-1-AP (uPA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

uPA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

uPA Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17968-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

uPA Fusion Protein Ag11813 (82-431 aa encoded by BC013575)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ASTDTMGRP CLPWNSATVL QQTYHAHRSD ALQLGLGKHN YCRNPDNRRR PWCYVQVGLK PLVQECMVHD CADGKKPSSP PEELKFQCGQ KTLRPRFKII GGEFTTIENQ PWFAAIYRRH RGGSVTYVCG GSLISPCWVI SATHCFIDYP KKEDYIVYLG RSRLNSNTQG EMKFEVENLI LHKDYSADTL AHHNDIALLK IRSKEGRCAQ PSRTIQTICL PSMYNDPQFG TSCEITGFGK ENSTDYLYPE QLKMTVVKLI SHRECQQPHY YGSEVTTKML CAADPQWKTD SCQGDSGGPL VCSLQGRMTL TGIVSWGRGC ALKDKPGVYT RVSHFLPWIR SHTKEENGLA L (82-431 aa encoded by BC013575)

Target Information

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Journal of Cancer

Guo ZC,Jing SL,Cui H,Xie LY,Na SJ,Tu JB

Mon Jun 19 00:00:00 UTC 2023

PLAU and LAMC2 can predict a poor prognosis in patients with HNSCC.

Journal of Cancer

Guo ZC,Jing SL,Cui H,Xie LY,Na SJ,Tu JB

Mon Jun 19 00:00:00 UTC 2023

PLAU contributes to the development of cholangiocarcinoma via activating NF-κB signaling pathway.

Cell biology international

Hu MD,Jia LH,Wang ML

Tue Aug 01 00:00:00 UTC 2023

Urokinase-type plasminogen activator blockade ameliorates experimental colitis in mice.

Scientific reports

Kida Y,Okahisa T,Sato Y,Bando M,Fujimoto S,Ma B,Nakagawa T,Kawaguchi T,Nakamura F,Okamoto K,Miyamoto H,Sogabe M,Tsuneyama K,Takayama T

Sat Feb 18 00:00:00 UTC 2023

Integrated analysis of Dendrobium nobile extract Dendrobin A against pancreatic ductal adenocarcinoma based on network pharmacology, bioinformatics, and validation experiments.

Frontiers in pharmacology

Xu X,Yu Y,Yang L,Wang B,Fan Y,Ruan B,Zhang X,Dai H,Mei W,Jie W,Zheng S

Tue Mar 21 00:00:00 UTC 2023

Integrated analysis of Dendrobium nobile extract Dendrobin A against pancreatic ductal adenocarcinoma based on network pharmacology, bioinformatics, and validation experiments.

Frontiers in pharmacology

Xu X,Yu Y,Yang L,Wang B,Fan Y,Ruan B,Zhang X,Dai H,Mei W,Jie W,Zheng S

Tue Mar 21 00:00:00 UTC 2023

Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma.

International journal of experimental pathology

Zeng C,Wu JK,Lu X

Thu Jun 01 00:00:00 UTC 2023

miR-3174 Is a New Tumor Suppressor MicroRNA That Inhibits Several Tumor-Promoting Genes in Glioblastoma.

International journal of molecular sciences

Hanif F,Zhang Y,Dube C,Gibert MK,Saha S,Hudson K,Marcinkiewicz P,Kefas B,Guessous F,Abounader R

Fri May 26 00:00:00 UTC 2023

Role of poFUT1 and O-fucosylation in placental angiogenesis†.

Biology of reproduction

Liang C,Li Y,Qin H,Ramzan MN,Wang H,Liu S,Yan Q

Tue Apr 11 00:00:00 UTC 2023

PDGFD switches on stem cell endothelial commitment.

Angiogenesis

Lu W,Xu P,Deng B,Zhang J,Zhan Y,Lin X,Xu X,Xia Z,Yang X,Zeng X,Huang L,Xie B,Wang C,Wang S,Kuang H,Han X,Mora A,Cao Y,Jiang Q,Li X

Tue Nov 01 00:00:00 UTC 2022

Macrophages are requisite for angiogenesis of type H vessels during bone regeneration in mice.

Bone

Kohara Y,Kitazawa R,Haraguchi R,Imai Y,Kitazawa S

Sat Jan 01 00:00:00 UTC 2022

Genetic insights into therapeutic targets for aortic aneurysms: A Mendelian randomization study.

EBioMedicine

Chen Y,Xu X,Wang L,Li K,Sun Y,Xiao L,Dai J,Huang M,Wang Y,Wang DW

Thu Sep 01 00:00:00 UTC 2022

PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice.

The Journal of experimental medicine

Cheng YW,Zhang ZB,Lan BD,Lin JR,Chen XH,Kong LR,Xu L,Ruan CC,Gao PJ

Mon Sep 06 00:00:00 UTC 2021

PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice.

The Journal of experimental medicine

Cheng YW,Zhang ZB,Lan BD,Lin JR,Chen XH,Kong LR,Xu L,Ruan CC,Gao PJ

Mon Sep 06 00:00:00 UTC 2021

Urokinase-type Plasminogen Activator Is a Therapeutic Target for Overcoming Sorafenib Resistance in Hepatoma Cells.

Anticancer research

Osawa M,Matsuda Y,Kinoshita Y,Wakai T

Mon Feb 01 00:00:00 UTC 2021

PLAU1 Facilitated Proliferation, Invasion, and Metastasis via Interaction With MMP1 in Head and Neck Squamous Carcinoma.

Frontiers in oncology

Wu K,Mao YY,Han NN,Wu H,Zhang S

Tue Apr 06 00:00:00 UTC 2021

The hepatocyte growth factor/c-met pathway is a key determinant of the fibrotic kidney local microenvironment.

iScience

Fu H,Gui Y,Liu S,Wang Y,Bastacky SI,Qiao Y,Zhang R,Bonin C,Hargis G,Yu Y,Kreutzer DL,Biswas PS,Zhou Y,Wang Y,Tian XJ,Liu Y,Zhou D

Fri Oct 22 00:00:00 UTC 2021

E4 engages uPAR and enolase-1 and activates urokinase to exert antifibrotic effects.

JCI insight

Sharma S,Watanabe T,Nishimoto T,Takihara T,Mlakar L,Nguyen XX,Sanderson M,Su Y,Chambers RA,Feghali-Bostwick C

Wed Dec 22 00:00:00 UTC 2021

RPL41 sensitizes retinoblastoma cells to chemotherapeutic drugs via ATF4 degradation.

Journal of cellular physiology

Geng W,Ren J,Shi H,Qin F,Xu X,Xiao S,Jiao Y,Wang A

Mon Mar 01 00:00:00 UTC 2021

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Science advances

Tan X,Banerjee P,Shi L,Xiao GY,Rodriguez BL,Grzeskowiak CL,Liu X,Yu J,Gibbons DL,Russell WK,Creighton CJ,Kurie JM

Tue Jun 01 00:00:00 UTC 2021

PLAU Promotes Cell Proliferation and Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma.

Frontiers in genetics

Chen G,Sun J,Xie M,Yu S,Tang Q,Chen L

Tue Jun 08 00:00:00 UTC 2021

PLAU Promotes Cell Proliferation and Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma.

Frontiers in genetics

Chen G,Sun J,Xie M,Yu S,Tang Q,Chen L

Tue Jun 08 00:00:00 UTC 2021

Identification of an Aging-Related Gene Signature in Predicting Prognosis and Indicating Tumor Immune Microenvironment in Breast Cancer.

Frontiers in oncology

Lv W,Zhao C,Tan Y,Hu W,Yu H,Zeng N,Zhang Q,Wu Y

Tue Jan 04 00:00:00 UTC 2022

Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes.

Aging

Zhou X,Li W,Yang J,Qi X,Chen Y,Yang H,Chu L

Sun Dec 26 00:00:00 UTC 2021

Transcriptome sequencing reveals fibrotic associated-genes involved in bovine mammary fibroblasts with Staphylococcus aureus.

The international journal of biochemistry & cell biology

Miao Z,Ding Y,Zhao N,Chen X,Cheng H,Wang J,Liu Y,Wang F

Wed Apr 01 00:00:00 UTC 2020

MiR-645 promotes invasiveness, metastasis and tumor growth in colorectal cancer by targeting EFNA5.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Li S,Hou X,Wu C,Han L,Li Q,Wang J,Luo S

Fri May 01 00:00:00 UTC 2020

NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.

Aging

Ma P,Li G,Jiang X,Shen X,Li H,Yang L,Liu W

Sat Dec 19 00:00:00 UTC 2020

MicroRNA-378-3p/5p suppresses the migration and invasiveness of oral squamous carcinoma cells by inhibiting KLK4 expression.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Cui Z,Sun S,Liu Q,Zhou X,Gao S,Peng P,Li Q

Wed Apr 01 00:00:00 UTC 2020

Curcumol may reverse early and advanced liver fibrogenesis through downregulating the uPA/uPAR pathway.

Phytotherapy research : PTR

Li G,Lin J,Peng Y,Qin K,Wen L,Zhao T,Feng Q

Mon Jun 01 00:00:00 UTC 2020

Rg3 inhibits gemcitabine-induced lung cancer cell invasiveness through ROS-dependent, NF-κB- and HIF-1α-mediated downregulation of PTX3.

Journal of cellular physiology

Ahmmed B,Kampo S,Khan M,Faqeer A,Kumar SP,Yulin L,Liu JW,Yan Q

Mon Jul 01 00:00:00 UTC 2019

Rg3 inhibits gemcitabine-induced lung cancer cell invasiveness through ROS-dependent, NF-κB- and HIF-1α-mediated downregulation of PTX3.

Journal of cellular physiology

Ahmmed B,Kampo S,Khan M,Faqeer A,Kumar SP,Yulin L,Liu JW,Yan Q

Mon Jul 01 00:00:00 UTC 2019

Sinulariolide Inhibits Gastric Cancer Cell Migration and Invasion through Downregulation of the EMT Process and Suppression of FAK/PI3K/AKT/mTOR and MAPKs Signaling Pathways.

Marine drugs

Wu YJ,Lin SH,Din ZH,Su JH,Liu CI

Wed Nov 27 00:00:00 UTC 2019

Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells.

International journal of biological macromolecules

Sun D,Liu Y,Wang H,Deng F,Zhang Y,Zhao S,Ma X,Wu H,Sun G

Sun Apr 01 00:00:00 UTC 2018

Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells.

International journal of biological macromolecules

Sun D,Liu Y,Wang H,Deng F,Zhang Y,Zhao S,Ma X,Wu H,Sun G

Sun Apr 01 00:00:00 UTC 2018

α-catenin SUMOylation increases IκBα stability and inhibits breast cancer progression.

Oncogenesis

Chen H,Xu Z,Li X,Yang Y,Li B,Li Y,Xia K,Wang J,Li S,Wang M,Wu H

Tue Mar 13 00:00:00 UTC 2018

α-catenin SUMOylation increases IκBα stability and inhibits breast cancer progression.

Oncogenesis

Chen H,Xu Z,Li X,Yang Y,Li B,Li Y,Xia K,Wang J,Li S,Wang M,Wu H

Tue Mar 13 00:00:00 UTC 2018

α-catenin SUMOylation increases IκBα stability and inhibits breast cancer progression.

Oncogenesis

Chen H,Xu Z,Li X,Yang Y,Li B,Li Y,Xia K,Wang J,Li S,Wang M,Wu H

Tue Mar 13 00:00:00 UTC 2018

α-catenin SUMOylation increases IκBα stability and inhibits breast cancer progression.

Oncogenesis

Chen H,Xu Z,Li X,Yang Y,Li B,Li Y,Xia K,Wang J,Li S,Wang M,Wu H

Tue Mar 13 00:00:00 UTC 2018

Evaluation of the Inhibitory Effects of Genipin on the Fluoxetine-Induced Invasive and Metastatic Model in Human HepG2 Cells.

Molecules (Basel, Switzerland)

Tian YS,Chen KC,Zulkefli ND,Maner RS,Hsieh CL

Fri Dec 14 00:00:00 UTC 2018

Evaluation of the Inhibitory Effects of Genipin on the Fluoxetine-Induced Invasive and Metastatic Model in Human HepG2 Cells.

Molecules (Basel, Switzerland)

Tian YS,Chen KC,Zulkefli ND,Maner RS,Hsieh CL

Fri Dec 14 00:00:00 UTC 2018

Adipocyte-derived exosomes promote lung cancer metastasis by increasing MMP9 activity via transferring MMP3 to lung cancer cells.

Oncotarget

Wang J,Wu Y,Guo J,Fei X,Yu L,Ma S

Tue Oct 10 00:00:00 UTC 2017

Ginkgolic acid inhibits the invasiveness of colon cancer cells through AMPK activation.

Oncology letters

Qiao L,Zheng J,Jin X,Wei G,Wang G,Sun X,Li X

Wed Nov 01 00:00:00 UTC 2017

Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells.

Scientific reports

Liu Y,Wang S,Sun D,Liu Y,Liu Y,Wang Y,Liu C,Wu H,Lv Y,Ren Y,Guo X,Sun G,Ma X

Tue Jul 19 00:00:00 UTC 2016

Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells.

Scientific reports

Liu Y,Wang S,Sun D,Liu Y,Liu Y,Wang Y,Liu C,Wu H,Lv Y,Ren Y,Guo X,Sun G,Ma X

Tue Jul 19 00:00:00 UTC 2016

Inhibition of plasmin protects against colitis in mice by suppressing matrix metalloproteinase 9-mediated cytokine release from myeloid cells.

Gastroenterology

Munakata S,Tashiro Y,Nishida C,Sato A,Komiyama H,Shimazu H,Dhahri D,Salama Y,Eiamboonsert S,Takeda K,Yagita H,Tsuda Y,Okada Y,Nakauchi H,Sakamoto K,Heissig B,Hattori K

Sun Mar 01 00:00:00 UTC 2015

Inhibition of plasmin protects against colitis in mice by suppressing matrix metalloproteinase 9-mediated cytokine release from myeloid cells.

Gastroenterology

Munakata S,Tashiro Y,Nishida C,Sato A,Komiyama H,Shimazu H,Dhahri D,Salama Y,Eiamboonsert S,Takeda K,Yagita H,Tsuda Y,Okada Y,Nakauchi H,Sakamoto K,Heissig B,Hattori K

Sun Mar 01 00:00:00 UTC 2015

Identification of potential targets for diallyl disulfide in human gastric cancer MGC-803 cells using proteomics approaches.

Oncology reports

Su B,Su J,He H,Wu Y,Xia H,Zeng X,Dai W,Ai X,Ling H,Jiang H,Su Q

Fri May 01 00:00:00 UTC 2015

Identification of potential targets for diallyl disulfide in human gastric cancer MGC-803 cells using proteomics approaches.

Oncology reports

Su B,Su J,He H,Wu Y,Xia H,Zeng X,Dai W,Ai X,Ling H,Jiang H,Su Q

Fri May 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: antagonist of uPA; CD87; HGNC:9052; plasminogen activator; plasminogen activator, urinary; U-plasminogen activator; uPA; UPAR; Urinary plasminogen activator, urokinase; URKR; Urokinase-type plasminogen activator

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Gene Aliases: ATF; BDPLT5; PLAU; QPD; u-PA; UPA; UPAM; URK

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UniProt ID: (Human) P00749, (Mouse) P06869

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Entrez Gene ID: (Human) 5328, (Rat) 25619, (Mouse) 18792

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Function(s)

serine-type endopeptidase activity protein binding peptidase activity kinase activity serine-type peptidase activity hydrolase activity

Process(es)

angiogenesis response to hypoxia proteolysis spermatogenesis embryo implantation positive regulation of cell proliferation regulation of receptor activity response to activity smooth muscle cell migration regulation of smooth muscle cell migration positive regulation of smooth muscle cell migration phosphorylation regulation of cell adhesion mediated by integrin response to hepatocyte growth factor cellular response to hepatocyte growth factor stimulus wound healing regulation of cell proliferation fibrinolysis skeletal muscle tissue regeneration response to hyperoxia positive regulation of ovulation neuron death cellular response to lipopolysaccharide cellular response to organic substance cellular response to glucose stimulus cellular response to hypoxia cellular response to fluid shear stress cellular response to staurosporine regulation of smooth muscle cell-matrix adhesion regulation of hepatocyte proliferation positive regulation of reactive oxygen species metabolic process chemotaxis signal transduction blood coagulation positive regulation of cell migration regulation of wound healing plasminogen activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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uPA Polyclonal Antibody

uPA Antibody (17968-1-AP) in ICC/IF

Product Details

17968-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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