Invitrogen

DNMT3B Polyclonal Antibody

DNMT3B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DNMT3B Antibody (PA1-884) in IHC (P)

Immunohistochemistry analysis of DNA Methyltransferase 3 showing positive staining in the nucleus and cytoplasm of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blo... View More

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Product Details

PA1-884

Applications

Tested Dilution

Publications

Western Blot (WB)

2.0 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2277463

Product Specific Information

PA1-884 detects DNA methyltransferase 3 from human and mouse tissues and cells as well as recombinant human Dnmt3. This antibody has been used to detect both recombinant and endogenous DNMT3b and recombinant DNMT3a.

PA1-884 has been successfully used in Immunohistochemistry (paraffin) and Western blot procedures. By Western blot, this antibody detects DNMT3 from P19 nuclear extracts.

The PA1-884 immunogen is a synthetic peptide corresponding to residues 1-14 of mouse DNMT3b. This sequence is 79% conserved between human and mouse. PA1-884 immunizing peptide (Cat. # PEP-117) is available for use in neutralization and control experiments.

Target Information

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DNA Methylation Dynamics During Esophageal Epithelial Regeneration Following Repair with Acellular Silk Fibroin Grafts in Rat.

Advanced biology

Urban LA,Li J,Gundogdu G,Trinh A,Shao H,Nguyen T,Mauney JR,Downing TL

PA1-884 was used in Immunohistochemistry to provide insight into the epigenetic mechanisms during esophageal regeneration following surgical repair with acellular grafts.

Mon May 01 00:00:00 UTC 2023

Decreases in different Dnmt3b activities drive distinct development of hematologic malignancies in mice.

The Journal of biological chemistry

Lopusna K,Nowialis P,Opavska J,Abraham A,Riva A,Haney SL,Opavsky R

PA1-884 was used in Western Blotting to study the Dnmt3b activity in vivo by generating Dnmt3b+/- mice expressing one wild-type allele as well as Dnmt3b+/CI and Dnmt3bCI/CI mice where one or both alleles express catalytically inactive Dnmt3bCI.

Tue Aug 31 00:00:00 UTC 2021

Decreases in different Dnmt3b activities drive distinct development of hematologic malignancies in mice.

The Journal of biological chemistry

Lopusna K,Nowialis P,Opavska J,Abraham A,Riva A,Haney SL,Opavsky R

Tue Aug 31 00:00:00 UTC 2021

Dnmt3b catalytic activity is critical for its tumour suppressor function in lymphomagenesis and is associated with c-Met oncogenic signalling.

EBioMedicine

Lopusna K,Nowialis P,Opavska J,Abraham A,Riva A,Opavsky R

PA1-884 was used in Western Blot to study a role of Dnmt3b's catalytic activity in development and cancer using a mouse model expressing catalytically inactive Dnmt3b (Dnmt3bCI).

Fri Jan 01 00:00:00 UTC 2021

Dnmt3b catalytic activity is critical for its tumour suppressor function in lymphomagenesis and is associated with c-Met oncogenic signalling.

EBioMedicine

Lopusna K,Nowialis P,Opavska J,Abraham A,Riva A,Opavsky R

PA1-884 was used in Western Blot to study a role of Dnmt3b's catalytic activity in development and cancer using a mouse model expressing catalytically inactive Dnmt3b (Dnmt3bCI).

Fri Jan 01 00:00:00 UTC 2021

Epigenetic Regulation of CDH1 Is Altered after HOXB7-Silencing in MDA-MB-468 Triple-Negative Breast Cancer Cells.

Genes

Paço A,Leitão-Castro J,Freitas R

PA1-884 was used in Western Blot to explore the contribution of HOXB7 deregulation to the aggressiveness of certain cancers such as triple-negative breast cancers.

Sun Oct 03 00:00:00 UTC 2021

Epigenetic Regulation of CDH1 Is Altered after HOXB7-Silencing in MDA-MB-468 Triple-Negative Breast Cancer Cells.

Genes

Paço A,Leitão-Castro J,Freitas R

PA1-884 was used in Western Blot to explore the contribution of HOXB7 deregulation to the aggressiveness of certain cancers such as triple-negative breast cancers.

Sun Oct 03 00:00:00 UTC 2021

Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions.

Nature communications

Nowialis P,Lopusna K,Opavska J,Haney SL,Abraham A,Sheng P,Riva A,Natarajan A,Guryanova O,Simpson M,Hlady R,Xie M,Opavsky R

PA1-884 was used in Western Blotting to highlight that Dnmt3b is a multifaceted protein that serves as an enzyme, an accessory factor for other methyltransferases, and as a transcriptional repressor in mouse embryogenesis.

Thu Sep 26 00:00:00 UTC 2019

Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages.

Nature communications

Yan J,Tie G,Wang S,Tutto A,DeMarco N,Khair L,Fazzio TG,Messina LM

PA1-884 was used in Western Blotting to demonstrate for the first time that type 2 diabetes mellitus induces an haematopoietic stem cell-autonomous mechanism that causes impaired wound healing.

Tue Jan 02 00:00:00 UTC 2018

The SNF2 family ATPase LSH promotes cell-autonomous de novo DNA methylation in somatic cells.

Nucleic acids research

Termanis A,Torrea N,Culley J,Kerr A,Ramsahoye B,Stancheva I

PA1-884 was used in western blot determine if the capacity for de novo DNA methylation of developmentally regulated loci is preserved in differentiated somatic cells

Mon Sep 19 00:00:00 UTC 2016

G9a/GLP Complex Maintains Imprinted DNA Methylation in Embryonic Stem Cells.

Cell reports

Zhang T,Termanis A,Özkan B,Bao XX,Culley J,de Lima Alves F,Rappsilber J,Ramsahoye B,Stancheva I

PA1-884 was used in western blot to characterize the G9a/GLP complex and how it maintains imprinted DNA methylation in embryonic stem cells

Tue Apr 05 00:00:00 UTC 2016

LSH and G9a/GLP complex are required for developmentally programmed DNA methylation.

Genome research

Myant K,Termanis A,Sundaram AY,Boe T,Li C,Merusi C,Burrage J,de Las Heras JI,Stancheva I

PA1-884 was used in western blot to investigate the role of LSH and G9a/GLP in DNA methylation during development

Sat Jan 01 00:00:00 UTC 2011

De novo DNA methyltransferase DNMT3b interacts with NEDD8-modified proteins.

The Journal of biological chemistry

Shamay M,Greenway M,Liao G,Ambinder RF,Hayward SD

PA1-884 was used in western blot to study the interaction between de novo DNA methyltransferase DNMT3b and NEDD8-modified proteins

Fri Nov 19 00:00:00 UTC 2010

LSH cooperates with DNA methyltransferases to repress transcription.

Molecular and cellular biology

Myant K,Stancheva I

PA1-884 was used in western blot to investigate the role of LSH during DNA methylation by DNA methyltransferases in mammals

Tue Jan 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: DNA (cytosine-5)-methyltransferase 3B; DNA (cytosine-5-)-methyltransferase 3 beta; DNA methyltransferase HsaIIIB; DNA methyltransferase MmuIIIB; DNA MTase HsaIIIB; DNA MTase MmuIIIB; Dnmt3; Dnmt3b; m.MmuIIIB; MGC1090

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Gene Aliases: DNMT3B; ICF; ICF1; M.HsaIIIB; MmuIIIB

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UniProt ID: (Human) Q9UBC3, (Mouse) O88509

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Entrez Gene ID: (Human) 1789, (Mouse) 13436

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Function(s)

chromatin binding transcription corepressor activity DNA (cytosine-5-)-methyltransferase activity protein binding DNA-methyltransferase activity histone deacetylase binding unmethylated CpG binding metal ion binding DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates DNA binding methyltransferase activity transferase activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter DNA methylation methylation-dependent chromatin silencing regulation of gene expression by genetic imprinting DNA methylation on cytosine within a CG sequence positive regulation of gene expression protein complex localization methylation positive regulation of neuron differentiation negative regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated S-adenosylhomocysteine metabolic process S-adenosylmethioninamine metabolic process positive regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation inactivation of X chromosome by DNA methylation cellular response to amino acid stimulus C-5 methylation of cytosine response to hypoxia response to toxic substance response to ionizing radiation response to activity response to caffeine response to estradiol response to vitamin A response to cocaine response to drug cellular response to hyperoxia cellular response to dexamethasone stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DNMT3B Polyclonal Antibody

DNMT3B Antibody (PA1-884) in IHC (P)

Product Details

PA1-884

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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