CD34 Antibody (63034942) in RE
Flow cytometry intrinsically facilitates the analysis of relative expression patterns in heterogeneous cell populations. Antibodies can be tested for staining in cell populations with known relative expression patterns. This representative image demonstrates the type of data that was used in the verification of specificity for this antibody. Legend: Normal human whole blood was surface stained with CD3 (clone UCHT1, left plot), HLA-DR (clone L243, middle plot), and CD16 (clone CB16, right plot). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis. As expected based on known expression patterns, the CD3 antibody only stains a subset of lymphocytes (T cells), the HLA-DR antibody stains monocytes and a subset of lymphocytes (B cells), and the CD16 antibody stains all granulocytes, a subset of monocytes and a subset of lymphocytes (NK cells).
