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Human SMAD1 (Total/Phospho) InstantOne™ ELISA Kit
Representative data for SMAD1 (Total/Phospho) Multispecies InstantOne™ ELISA. C2C12 cells were either inhibited (50 mM dosomorphin for 60 min) (-) or stimulated with BMP4 (10 ng/mL for 30 min) (+). Lysates were prepared as described in the InstantOne™ User Manual. Cells were analyzed for total (left) or phosphorylated (right) SMAD1 by InstantOne™ ELISA using 10 µg/analysis.
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
SMAD1
Target sub-specificity
Total
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human SMAD1 (Ser463/465) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
SMAD1 is a member of a family of transcription factors involved in mediating signaling by growth and differentiation factors such as transforming growth factor beta (TGF-b) and bone morphogenic protein (BMP).
Target information
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1, SMAD1
Gene ID : (Human) 4086
Gene symbol : SMAD1
Protein Aliases : BSP-1, DWFC, hSMAD1, JV5-1, MADH5, Mothers-against-DPP-related-1, OTTHUMP00000220456, SMAD5, SMAD, JV4-1, MAD homolog 1, MAD, mothers against decapentaplegic homolog 1, Mad-related protein 1, Mothers against decapentaplegic homolog 1, mothers against DPP homolog 1, SMAD 1, SMAD family member 1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1, Transforming growth factor-beta-signaling protein 1
UniProt ID (Human) Q15797
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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