Amplification of the GFP signal localized in mitochondria of a transfected HeLa cell.

HeLa cell transfected with pShooter pCMV/myc/mito/GFP, then fixed and permeabilized. Green-fluorescent protein (GFP) localized in the mitochondria was labeled with anti-GFP mouse IgG2a (Cat. no. A11120) and detected with orange-fluorescent Alexa Fluor® 555 goat anti–mouse IgG (CAt. no. A21422), which colocalized with the dim GFP fluorescence. F-actin was labeled with green-fluorescent Alexa Fluor® 488 phalloidin (Cat. no. A12379), and the nucleus was stained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490). The sample was mounted using ProLong® Gold antifade reagent (Cat. no. P36930). Some GFP fluorescence is retained in the mitochondria after fixation (top), but immunolabeling and detection greatly improve visualization (bottom).

HeLa cell transfected with pShooter pCMV/myc/mito/GFP, then fixed and permeabilized. Green-fluorescent protein (GFP) localized in the mitochondria was labeled with anti-GFP mouse IgG<sub>2a</sub> (Cat. no. A11120) and detected with orange-fluorescent Alexa Fluor® 555 goat anti–mouse IgG (CAt. no. A21422), which colocalized with the dim GFP fluorescence. F-actin was labeled with green-fluorescent Alexa Fluor® 488 phalloidin (Cat. no. A12379), and the nucleus was stained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490). The sample was mounted using ProLong® Gold antifade reagent (Cat. no. P36930). Some GFP fluorescence is retained in the mitochondria after fixation (top), but immunolabeling and detection greatly improve visualization (bottom).

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