Citrate Buffer (pH 6.0), Concentrate
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Citrate Buffer (pH 6.0), Concentrate

Citrate buffer is used in Heat Induced Epitope Retrieval (HIER) methods. HIER is used to reverse the loss of antigenicityRead more
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Catalog number 005000
Price (USD)
140.00
Each
Add to cart
Price (USD)
140.00
Each
Add to cart
Citrate buffer is used in Heat Induced Epitope Retrieval (HIER) methods. HIER is used to reverse the loss of antigenicity that occurs with some epitopes in formalin-fixed paraffin embedded tissues. During epitope retrieval, tissue slides are immersed in a solution and heated (>95° C) by hot plate or other appropriate heat source such as a pressure cooker or steamer. Citrate buffer is the preferred solution for most antibodies.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialCitrate Buffer
Recommended StorageStore in refrigerator (2–8°C).

Shelf life: 18 months from date of manufacture
pH6
Quantity100 mL
Solution TypeAntigen Retrieval Reagent
Unit SizeEach

Frequently asked questions (FAQs)

What is the concentration/molarity of the Citrate Buffer (pH 6.0), Concentrate (Cat. No. 005000)?

The Citrate Buffer (pH 6.0), Concentrate (Cat. No. 005000) is a 20X solution and contains two compounds with different molecular weights, so there is no single molarity for the formulation. The citric acid is at about 20 mM and sodium citrate is at about 180 mM.

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