OneComp eBeads™ Compensation Beads
OneComp eBeads™ Compensation Beads
Invitrogen™

OneComp eBeads™ Compensation Beads

OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. The beads are sphericalRead more
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Catalog NumberQuantity
01-1111-4125 tests
01-1111-42100 tests
Catalog number 01-1111-41
Price (USD)
132.00
Each
Add to cart
Quantity:
25 tests
Price (USD)
132.00
Each
Add to cart
OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. The beads are spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls.

Each drop of beads contains two populations: a positive population that will capture any mouse, rat or hamster antibody and a negative population that will not react with antibody. When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations result. This bimodal distribution can be used for single-color compensation controls in multicolor flow cytometry experiments.

OneComp eBeads cross-react to some antibodies of rabbit origin, but have not been extensively tested for this reactivity. OneComp eBeads are designed for use in compensation with all fluorochromes excited by blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633-635 nm) lasers. This product is compatible with eFluor™ 450 but is not optimized for compensation of other fluorochromes excited by a violet (405 nm) laser.

Reactivity/Species
Hamster, Mouse, Rat

Reported Application
Flow Cytometric Analysis

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Flow Cytometer
No. of Tests25 tests
Quantity25 tests
Product LineeBeads™
Product TypeCompensation Beads
Unit SizeEach

Frequently asked questions (FAQs)

I am performing flow cytometry. My compensation matrix has values over 100. That's not possible is it?

It is possible. But if you have a lot of values over 100, you probably need to look at the voltages that you are using for your data collection.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I’ve been told that I need to compensate my data for flow cytometry analysis. What does that mean?

By running single-color controls, it is possible to remove signal of one fluorophore that spills over into the collection channel for another fluorophore.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kind of controls do I need for flow cytometry?

For compensation, you need to prepare a singly stained sample (or compensation beads) for each color parameter that you are using. In addition, we recommend that you use FMO (flow minus one) controls. These are controls in which you label cells or beads with every color in your panel, omitting one. Make one FMO control for each color. These controls are important for helping you properly set gates on your data.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why should I worry about compensation in flow cytometry analysis?

In a perfect world, the fluorescence emission profile for each individual fluorophore would be a very intense, narrow peak, well separated from all other emission peaks. In reality, organic dyes and fluorescent proteins have broad emission peaks, and compensation must be employed (during or after data acquisition) to correctly assign fluorescence signal to each fluorophore. Compensation is important because it removes fluorescent signal from overlapping spectra so you know that the signal you see is only the signal from the fluorophore of interest.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I make compensation controls for antibodies?

All that you need for compensation controls is an unstained sample of your cells (or negative control beads) and samples with each of your fluorophores - one per tube. You want the single-stained controls to be as bright as you expect your brightest sample to be. Antibodies can be bound to beads instead of cells using our AbC Total Antibody Compensation Bead Kit (Cat. Nos. A10513 and A10497).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all OneComp eBeads™ Compensation Beads FAQs