PBS, pH 7.4
PBS, pH 7.4
Gibco™

PBS, pH 7.4

PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washingRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
10010023500 mL
100100311000 mL
1001004910 x 500 mL
100100726 x 1000 mL
Catalog number 10010023
Price (USD)
30.74
Each
Add to cart
Quantity:
500 mL
Price (USD)
30.74
Each
Add to cart

PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. We offer a variety of PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.


This PBS is manufactured as follows:
Without
• Calcium
• Magnesium
• Phenol Red


The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AdditiveNo Sodium Pyruvate
ClassificationAnimal Origin-Free
ColorClear
Dilution1 X
Product LineGibco™
Product TypePhosphate Buffered Saline (PBS)
Quantity500 mL
Solution TypePhosphate Buffered Saline
For Use With (Application)Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue
Green FeaturesSustainable packaging
Osmolality280 - 315 mOsm/kg
pH7.4
Phenol Red IndicatorNo Phenol Red
Shipping ConditionRoom Temperature
SterilitySterile-filtered
FormLiquid
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Unit SizeEach
Contents & Storage
Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

Frequently asked questions (FAQs)

How can I make isolation buffer for cell isolation?

To prepare this buffer:

(1) Make up 100 mL of a stock solution of 0.5 M EDTA by dissolving 14.6 g EDTA in approximately 80 mL of ddH2O. Adjust the pH to 7.4, then adjust the volume to 100 mL. Autoclave and filter 25 mL aliquots through a sterilized filter into 4 sterile 50 mL tubes. Store one at 4 degrees C and the rest at -20 degrees C for future use.
(2) Make up a stock solution of 20% BSA by putting 5 g of BSA in a 50 mL tube and filling it with ddH2O up to 25 mL. Leave on a roller at room temperature until dissolved. Filter through a sterile 0.2 µm filter into a sterile 50 mL tube and adjust the volume to 30 mL with sterile H2O.
(3) Make the isolation buffer by taking a 500 mL bottle of 1x PBS, pH 7.4 without Ca2+ or Mg2+ (Cat. No. 10010023). Add 2.5 mL 20% BSA and 2 mL 0.5 M EDTA. Mix by turning the bottle end over end 10 times. Store at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My PBS, PH 7.4 (Cat. No. 10010072) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products tested for endotoxin levels?

Our PBS, pH 7.2 and PBS, pH 7.4 products are tested for endotoxin and the endotoxin levels are shown on the Certificate of Analysis (CoA) for the particular lot number of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products sterile and suitable for cell culture?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625), which is molecular biology grade and is certified to be RNase-free. However, it is not sterile and hence is not cell culture-grade.

All our other PBS products (Cat. Nos. 70011xxx, 10010xxx, 70013xxx, 20012xxx) are filter-sterilized using a 0.1 µm filter and are cell culture grade (ready to use directly on cells). However, they are not tested for nuclease activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products DNase- and RNase-free?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625) that is molecular biology grade and is certified to be RNase-free. Our other PBS products are made with distilled water and are filter-sterilized, but are not tested for RNase and DNase activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.