Taq DNA Polymerase, recombinant
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<i>Taq</i> DNA Polymerase, recombinant
<i>Taq</i> DNA Polymerase, recombinant
<i>Taq</i> DNA Polymerase, recombinant
Invitrogen™

Taq DNA Polymerase, recombinant

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
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Catalog NumberNo. of Reactions
10342020500 Reactions
10342053100 Reactions
103420461500 Reactions
103421785000 Reactions
Catalog number 10342020
Price (USD)
334.00
Each
Add to cart
No. of Reactions:
500 Reactions
Request bulk or custom format
Price (USD)
334.00
Each
Add to cart
Taq DNA Polymerase, recombinant, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.

Features

  • A choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • Enzyme is licensed and qualified for PCR

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • Sequencing ssDNA
  • Cycle sequencing

Notes

  • One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
  • For superior PCR performance, DreamTaq DNA Polymerase is recommended.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone enzyme
GC-Rich PCR PerformanceLow
PCR MethodqPCR, Standard PCR
PolymeraseTaq DNA Polymerase
Reaction SpeedStandard
Exonuclease Activity5' - 3'
Product TypeDNA Polymerase
Quantity500 Units
Shipping ConditionDry Ice
For Use With (Application)Standard PCR
Concentration5 U/μL
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions500 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Starting MaterialDNA
Unit SizeEach
Contents & Storage
• Taq DNA Polymerase (5 U/μL), 100 μL
• 10X PCR buffer, 2.5 mL
• 50 mM MgCl2, 1 mL

Store at -10°C to -30°C in a non-frost-free freezer.
Guaranteed stable for 6 months when stored properly.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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