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Gibco™
MCDB 131 Medium, no glutamine
MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascularRead more
Catalog number 10372019
Price (USD)
78.00
Each
Price (USD)
78.00
Each
MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.
This MCDB 131 Medium is manufactured as follows:
The complete formulation is available.
Using MCDB 131
MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Dual-site cGMP manufacturing
For supply chain continuity, we manufacture MCDB 131 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
This MCDB 131 Medium is manufactured as follows:
| With | Without |
| • Phenol Red | • L-glutamine |
| • Sodium pyruvate | • HEPES |
The complete formulation is available.
Using MCDB 131
MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Dual-site cGMP manufacturing
For supply chain continuity, we manufacture MCDB 131 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeHuman Microvascular Endothelial Cells (HMVEC), Human Omental Microvascular Cells, Hepatocytes, Myocytes, Smooth Muscle Cells
Concentration1 X
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeMCDB 131 Medium
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesLow Glucose, Phenol Red, Sodium Pyruvate
Without AdditivesNo Glutamine, No HEPES
Green FeaturesSustainable packaging
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 2–8°C (protect from light)
Shelf life: 12 months from date of manufacture
Shelf life: 12 months from date of manufacture