Penicillin-Streptomycin-Glutamine (100X)
Penicillin-Streptomycin-Glutamine (100X)
Gibco™

Penicillin-Streptomycin-Glutamine (100X)

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination in cell cultures due to their effective combined actionRead more
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Catalog number 10378016
Price (USD)
42.81
Each
Add to cart
Price (USD)
42.81
Each
Add to cart
The antibiotics penicillin and streptomycin are used to prevent bacterial contamination in cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . It acts by binding to the 30S subunit of the bacterial ribosome, leading to the inhibition of protein synthesis and death in susceptible bacteria. This solution contains 10,000 units of penicillin, 10,000 μg of streptomycin, and 29.2 mg/ml of L-glutamine in a 10 mM citrate buffer (for pH stability).

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats. See the complete list, or find products for:
• Contamination control
• Eukaryotic and bacterial selection

See recommended working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration100 X, 50 mg/mL
FormLiquid
Product TypeAntibiotic-L-Glutamine
Shelf Life12 Months
SterilitySterile-filtered
With AdditivesGlutamine
For Use With (Application)Prevention of Cell Culture Contamination
Quantity100 mL
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.