Exosome-Human CD9 Isolation Reagent (from cell culture)
Exosome-Human CD9 Isolation Reagent (from cell culture)
Invitrogen™

Exosome-Human CD9 Isolation Reagent (from cell culture)

Exosome-Human CD9 Isolation Reagent (from cell culture) enables isolation of CD9-positive extracellular vesicles (ECVs) such as exosomes from enriched cellRead more
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Catalog number 10614D
Price (USD)
634.00
Each
Add to cart
Price (USD)
634.00
Each
Add to cart

Exosome-Human CD9 Isolation Reagent (from cell culture) enables isolation of CD9-positive extracellular vesicles (ECVs) such as exosomes from enriched cell culture media. Following isolation, the specific sub-population of exosomes/ECVs can be further studied using methods like Western blotting, qPCR, or next generation sequencing.

• Minimal hands-on time for specific exosome isolation
• Obtain a highly pure exosome subset
• Protocol is easily scalable
• Compatible with most downstream analysis

Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads™ magnetic separation technology, these subsets can be selectively isolated and studied. The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation. Following enrichment, specific exosomes are isolated by binding to the surface of the magnetic beads.

Dynabeads™ magnetic beads are known for their sensitivity, reproducibility, and stability. Dynabeads™ magnetic separation technology also allows you to 'see' your sample during handling due to the color of the magnetic beads. When the sample tube is placed in the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and buffer changes. In addition, the sample and bead volume can be easily scaled according to your sample size or downstream applications.

Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.

For research use only. Not for use in diagnostic procedures.
Specifications
Product TypeIsolation Reagent
Product LineDynabeads™
Quantity2 mL
Sample TypeCell Cultures
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).

Frequently asked questions (FAQs)

Do you have any references or citations using Dynabeads magnetic beads to isolate exosomes?

Yes. See this poster (https://tools.thermofisher.com/content/sfs/posters/Exosome-poster-ISEV-2013-Boston.pdf).

In addition, here are some citations:
- Blood 91:2573 (1998)
- Science 289:444 (2000)
- J Physiol 537:537 (2001)
- Mol Cell Proteomics 12:587 (2013)
- Biol Reprod 81:717 (2009)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can exosomes isolated with different surface markers be different?

Yes, exosomes isolated with different surface markers can be distinctive in their protein profile. This has been demonstrated by Tauro et al. (http://www.ncbi.nlm.nih.gov/pubmed/23230278), who isolated two distinctive populations of exosomes based on surface markers EpCam or A33 from conditioned cell culture medium from a human carcinoma cell line. This proteomics study indicated that these two populations of exosomes are unique.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kind of product do you suggest if I would like to use Dynabeads magnetic beads to isolate exosomes?

We have exosome isolation kits for Exosome-Human CD63 (Cat. No. 10606D), Exosome-Human CD9 (Cat. No. 10614D), Exosome-Human CD81 (Cat. No. 10616D), and Exosome-Human EpCAM intended for isolating exosomes with these commonly used exosome surface antigens. If you are interested to isolating exosomes with other specific surface markers using your own antibody, you can use our Dynabeads exosome immunoprecipitation (Protein A, Cat. No. 10610D), Dynabeads exosome immunoprecipitation (Protein G, Cat. No. 10612D), or Exosome-Streptavidin for isolation/detection (Cat. No. 10608D). In addition, anti-mouse IgG Dynabeads magnetic beads (Cat Nos. 11031 or 11033) also can be employed in exosome isolation using mouse monoclonal antibodies against selected surface markers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How are exosomes characterized?

Exosomes are usually characterized by flow cytometry (using surface markers such as CD9, CD63, TSG101, and Alix), by EM to study morphology and size, or by detailed protein analysis by LC-MS/MS.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the commonly used surface markers for isolating exosomes?

It depends on the cell source from which the exosomes were derived. The most commonly used surface markers for isolating and characterizing exosomes are CD9, CD63, CD81, or TSG101. Here are some of the recent references and surface markers for identifying or isolating exosomes:

Alix, CD63, EpiCam, HSP70, TSG101 Mol Cell Proteomics 12:587 (2013)
CD9, CD63 Hum Mol Genet 21:R125 (2012)
CD63, MHC IIJ Biol Chem 278:52347 (2003)
CD9, CD81, Lamp1, TSG101 Cancer Res 67:7458 (2007)
CD63 Nature Cell Biol 9:654 (2007)
Alix, CD37, CD53, CD63, CD81, CD82, TSG101J Cell Biol 200:373 (2013)
CD59, CD63, CD133, TSG101 FASEB J 23:1858 (2009)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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