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RNaseOUT™ Recombinant Ribonuclease Inhibitor
RNaseOUT™ Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used toRead more
Catalog number 10777019
Price (USD)
237.00
Each
Price (USD)
237.00
Each
RNaseOUT™ Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used to avoid RNA degradation in a variety of applications. RNaseOUT™ Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of ∼52 kDa. RNaseOUT™ inhibits RNase A, RNase B, and RNase C.
Applications
cDNA synthesis, RT-PCR, and in vitro transcription and translation
Source
Purified by affinity chromatography from E. coli expressing a cloned porcine gene
Performance and quality testing
SDS-PAGE purity, endodeoxyribonuclease assay, protein concentration, specific activity, performance evaluated by RT-PCR
Unit definition
One unit inhibits 5 ng of RNase A by 50% using cytidine 2´, 3´ cyclic monophosphate (cCMP) as the substrate
Unit reaction conditions
100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A in 1 mL from 0 to 10 min at 25°C
Applications
cDNA synthesis, RT-PCR, and in vitro transcription and translation
Source
Purified by affinity chromatography from E. coli expressing a cloned porcine gene
Performance and quality testing
SDS-PAGE purity, endodeoxyribonuclease assay, protein concentration, specific activity, performance evaluated by RT-PCR
Unit definition
One unit inhibits 5 ng of RNase A by 50% using cytidine 2´, 3´ cyclic monophosphate (cCMP) as the substrate
Unit reaction conditions
100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A in 1 mL from 0 to 10 min at 25°C
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration100 mM
Purification Time10 min.
Product LineRNaseOUT™
Quantity5000 units
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Store at -20°C. Avoid exposing product to frequent changes in temperature. RNaseOUT™ Ribonuclease Inhibitor requires 1 mM DTT to maintain activity.
The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation.
The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation.