Platinum™ Taq DNA Polymerase
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For superior PCR performance, upgrade to Invitrogen's new Platinum II Taq Hot-start DNA Polymerase.
Platinum&trade; <i>Taq</i> DNA Polymerase
Invitrogen™

Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
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Catalog NumberNo. of Reactions
10966034600 Reactions
10966018120 Reactions
10966026300 Reactions
109660835000 Reactions
Catalog number 10966034
Price (USD)
770.00
Each
Add to cart
No. of Reactions:
600 Reactions
Request bulk or custom format
Price (USD)
770.00
Each
Add to cart
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot-start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.

The hot-start property allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.

Features

  • Convenient reaction assembly at room temperature
  • 5' to 3' exonuclease activity
  • Lacks 3' to 5' exonuclease activity

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • Hot-start PCR
  • RT-PCR

Notes

  • For superior PCR performance, the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase is recommended.
  • Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatTube
GC-Rich PCR PerformanceLow
PolymerasePlatinum Taq DNA Polymerase
Reaction SpeedFast or Standard
Exonuclease Activity5' - 3'
Product TypeDNA Polymerase
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR
Fidelity (vs. Taq)1X
Hot StartBuilt-In Hot Start
No. of Reactions600 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Starting MaterialDNA
Unit SizeEach
Contents & Storage
• Platinum Taq DNA Polymerase, 120 μL
• 10X PCR Buffer (without magnesium), 3 x 1.25 mL
• 50 mM MgCl2, 1 mL
• KB Extender, 1.3 mL

Store at -10°C to -30°C in a non-frost-free freezer.

Frequently asked questions (FAQs)

Can I purchase just the 10X PCR buffer that comes with Platinum Taq?

The 10X PCR buffer for Platinum Taq is not available as a stand-alone item. It is only supplied as part of the enzyme kit.

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

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