Medium 199, Earle's Salts
Medium 199, Earle's Salts
Gibco™

Medium 199, Earle's Salts

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivationRead more
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Catalog NumberQuantity
11150059500 mL
31150022500 mL
1115006710 x 500 mL
Catalog number 11150059
Price (USD)
39.28
Each
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Quantity:
500 mL
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Price (USD)
39.28
Each
Add to cart

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This Medium 199 is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red 


The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineRat epithelial
Cell TypeChick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
ClassificationAnimal Origin
Concentration1X
FormLiquid
Product TypeMedium 199
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesLow Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 2–8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

We do not provide stability data for the product once it is opened as it would depend on the usage and storage conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Medium 199, Earle's Salts FAQs