Dynabeads™ M-280 Sheep Anti-Mouse IgG
Inquire about OEM or Commercial Supply version of this product here.
Dynabeads™ M-280 Sheep Anti-Mouse IgG
Invitrogen™

Dynabeads™ M-280 Sheep Anti-Mouse IgG

Dynabeads M-280 Sheep anti-Mouse IgG beads are 2.8-μm monosized, monodispersed, superparamagnetic beads with affinity-purified polyclonal sheep anti-mouse IgG covalently boundRead more
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Catalog NumberVolume (Metric)
11201D2 mL
11202D10 mL
Catalog number 11201D
Price (USD)
534.00
Each
Add to cart
Volume (Metric):
2 mL
Price (USD)
534.00
Each
Add to cart

Dynabeads M-280 Sheep anti-Mouse IgG beads are 2.8-μm monosized, monodispersed, superparamagnetic beads with affinity-purified polyclonal sheep anti-mouse IgG covalently bound to the bead surface. These beads are intended for use as a solid support for simple and efficient binding of mouse IgGs and their target proteins. The uniform, monodisperse, and non-porous Dynabeads magnetic beads make them an ideal choice for applications such as binding immunoglobulins, protein purification, sandwich immunoassays, immunoprecipitation (IP), Co-immunoprecipitation (Co-IP), and isolation of cells and microorganisms. The polyclonal sheep antibody binds mouse IgG1, IgG2a, and IgG2b, and, with a lower reactivity, mouse IgG3 and IgM. Human cross-reactivity is minimal.

Benefits of Dynabeads M-280 Sheep anti-Mouse IgG beads include:
• Speed—isolate protein in less than 40 minutes
• Selectivity—binds most mouse IgG subclasses, very low non-specific binding
• Flexibility—no columns, centrifugations, or time-consuming pre-clearing required
• Automation ready—automation protocols available with the KingFisher Sample Purification instruments

Great signal to noise ratio
Dynabeads M-280 Sheep anti-Mouse IgG beads covalently couple the secondary sheep antibodies to a hydrophilic surface, which means the non-specific hydrophobic interactions are reduced to a minimum. Since this interaction is the main driver of unwanted protein binding, Dynabeads magnetic beads help ensure a great signal-to-noise ratio. This Dynabeads magnetic bead platform binds any mouse IgG antibody in a solution and can therefore become a powerful tool in affinity purification.

Dynabeads immunoprecipitation protocol is fast and easy
Add the target specific primary mouse IgG antibody directly to the sample (indirect technique) or first pre-coat the target antibody onto the Dynabeads M-280 Sheep Anti-Mouse IgG beads (direct technique). Regardless of which technique is used, when the Dynabeads M-280 Sheep anti-Mouse IgG beads are mixed with the sample, they bind to the target. The manual IP protocol is simple and can be performed in less than 40 minutes with 3 easy bind-incubate-elute steps. The procedure does not require any pre-clearing due to the low non-specific binding and uses very little antibody while still obtaining a high IP yield, thus saving both time and cost per IP.

The antibody-coated beads can be used in a variety of IP applications such as Co-IP, chromatin IP (ChIP/ChIP sequencing), RNA IP (RIP, RIP sequencing), small-scale IgG purification, and protein purification. The isolated pure target protein can be used in downstream western blot, mass spectrometry analysis, sequencing, etc.

Automation-ready for use with KingFisher purification systems
The 2.8-μm magnetic beads are ideal for high-throughput enrichment and can be automated using any of our KingFisher sample purification systems

Commercial supply
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Sheep anti-Mouse IgG beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.

Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems:
• See our immunoprecipitation selection table, data, and references
• Dynabeads magnetic beads selection guide
• See magnets for Dynabeads magnetic beads separations
• KingFisher automation protocols
• Watch a video about the KingFisher Flex instrument

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibody FragmentWhole Antibody
Certifications/ComplianceISO9001 and ISO13485
Concentration10 mg/mL
ContentsDynabeads M-280 Sheep Anti-Mouse IgG is supplied in PBS, pH 7.4 w/0.1% BSA, 0.02% NaN3
DescriptionPolyclonal sheep anti-mouse IgG covalently bound to Dynabeads
ColorBrown
For Use With (Application)Immunoprecipitation, Protein Purification
MaterialPolystyrene
Product TypeAntibody Coated Bead
FormatBeads in suspension
For Use With (Equipment)KingFisher™ Sample Purification Systems, DynaMag™ Magnets
High-throughput CompatibilityHigh-throughput Compatible
Host SpeciesSheep
Isolation TechnologyMagnetic Beads
Ligand TypeAntibody
Preservative0.02% NaN3
Purification TargetProteins
ReactivityBinds mouse IgG1, IgG2a and IgG2b. Low reactivity towards mouse IgG3 and IgM.
Shelf Life36 months from date of manufacture
Shipping ConditionAmbient temperature
Target SpeciesMouse IgG's
UniformityMonosized 2.8 μm (CV <5%)
Volume (Metric)2 mL
Diameter (Metric)2.8 μm
Regulatory StatusFor Research Use Only
Unit SizeEach
Contents & Storage
Store at 2°C to 8°C.

Frequently asked questions (FAQs)

I am getting high background using Dynabeads M-280 Streptavidin magnetic beads. How can I prevent this from happening?

The background might be caused by nonspecific binding to the BSA on the bead surface. Alternatively, high background might be caused by nonspecific binding to streptavidin. Increasing either the pH or the salt concentration might help reduce the binding. Dynabeads M-270 Streptavidin magnetic beads might be a better alternative; these beads are not coated with BSA and are hydrophilic, as they are based upon carboxylic acid chemistry.

My Dynabeads magnetic beads are not pelleting well with the magnet. Do you have any suggestions for me?

Please review the following possibilities for why your Dynabeads magnetic beads are not pelleting:

- The solution is too viscous.
- The beads have formed aggregates because of protein-protein interaction.

Try these suggestions: - Increase separation time (leave tub on magnet for 2-5 minutes)
- Add DNase I to the lysate (~0.01 mg/mL)
- Increase the Tween 20 concentration to ~0.05% of the binding and/or washing buffer.
- Add up to 20 mM beta-merecaptoethanol to the binding and/or wash buffers.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

I have a long double-stranded DNA fragment I would like to isolate. What product do you recommend?

For biotin-labeled DNA that is less than 1 kb, we recommend you use Dynabeads M270 Streptavidin (Cat. No. 65305) and MyOne C1 magnetic beads (Cat. No. 65001). We recommend our Dynabeads KilobaseBINDER Kit (Cat. No. 60101), which is designed to immobilize long (>1 kb) double-stranded DNA molecules. The KilobaseBINDER reagent consists of M-280 Streptavidin-coupled Dynabeads magnetic beads along with a patented immobilization activator in the binding solution to bind to long, biotinylated DNA molecules for isolation. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/immobilisation-of-long-biotinylated-dna-fragments.html) for more information in regards to long biotinylated DNA fragment isolation.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

Can I use Dynabeads magnetic beads to isolate single-stranded DNA templates?

Yes, Dynabeads magnetic beads can be used to isolate single-stranded DNA. Streptavidin Dynabeads magnetic beads can be used to target biotinylated DNA fragments, followed by denaturation of the double-stranded DNA and removal of the non-biotinylated strand. The streptavidin-coupled Dynabeads magnetic beads will not inhibit any enzymatic activity. This enables further handling and manipulation of the bead-bound DNA directly on the solid phase. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/preparing-single-stranded-dna-templates.html) for more information in regards to single-stranded DNA capture.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

What is the magnetic susceptibility for Dynabeads magnetic beads?

Magnetic susceptibility is a measure of how quickly the beads will migrate to the magnet. This will depend on the iron content and the character of the iron oxide. The magnetic susceptibility given for the Dynabeads magnetic beads is the mass susceptibility, given either as cgs units/g or m^3/kg (the latter being an SI unit). For ferri- and ferromagnetic substances, the magnetic mass susceptibility is dependent upon the magnetic field strength (H), as the magnetization of such substances is not a linear function of H but approaches a saturation value with increasing field. For that reason, the magnetic mass susceptibility of the Dynabeads magnetic beads is determined by a standardized procedure under fixed conditions. The magnetic mass susceptibility given in our catalog is thus the SI unit. Conversion from Gaussian (cgs, emu) units into SI units for magnetic mass susceptibility is achieved by multiplying the Gaussian factor (emu/g or cgs/g) by 4 pi x 10^-3. The resulting unit is also called the rationalized magnetic mass susceptibility, which should be distinguished from the (SI) dimensionless magnetic susceptibility unit. In general, magnetic mass susceptibility is a measure of the force (Fz) influencing an object positioned in a nonhomogenous magnetic field. The magnetic mass susceptibility of the Dynabeads magnetic beads is measured by weighing a sample, and then subjecting the sample to a magnetic field of known strength. The weight (F1) is then measured, and compared to the weight of the sample when the magnetic field is turned off (F0). The susceptibility is then calculated as K x 10^-3 = [(F1-F0) x m x 0.335 x 10^6], where K is the mass susceptibility of the sample of mass m. The susceptibility is then converted to SI units.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

View all Dynabeads™ M-280 Sheep Anti-Mouse IgG FAQs