Dynabeads™ M-280 Sheep Anti-Rabbit IgG
Dynabeads™ M-280 Sheep Anti-Rabbit IgG
Invitrogen™

Dynabeads™ M-280 Sheep Anti-Rabbit IgG

Dynabeads M-280 Sheep Anti-Rabbit IgG are 2.8 μm superparamagnetic beads with affinity-purified polyclonal sheep anti-rabbit IgG covalently bound to theRead more
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Catalog NumberVolume (Metric)
11203D2 mL
11204D10 mL
Catalog number 11203D
Price (USD)
534.00
Each
Add to cart
Volume (Metric):
2 mL
Price (USD)
534.00
Each
Add to cart

Dynabeads M-280 Sheep Anti-Rabbit IgG are 2.8 μm superparamagnetic beads with affinity-purified polyclonal sheep anti-rabbit IgG covalently bound to the bead surface. These beads are designed to serve as a solid support for simple and efficient binding of rabbit IgGs of all subclasses and their target proteins. The uniform, monodisperse, and non-porous Dynabeads make them an ideal choice for applications such as binding Ig, protein purification, sandwich immunoassays, immunoprecipitation (IP), Co-IP, and isolation of cells and microorganisms.

• Isolate protein in less than 40 minutes
• Binds all rabbit IgG subclasses
• Very low non-specific binding with high signal-to-noise ratio
• No columns, centrifugations, or time-consuming pre-clearing required
• High reproducibility and high throughput compatible with KingFisher instruments

Manual Dynabeads separation is fast and easy to perform
The primary antibody that recognizes the target molecule may be added to the sample (indirect technique) or pre-coated onto the Dynabeads M-280 Sheep Anti-Rabbit IgG (direct technique). Regardless of which technique is used, when the Dynabeads M-280 Sheep Anti-Rabbit IgG are mixed with the sample, the beads bind to the target. Placing the sample on a DynaMag magnet separates the bead-bound target from the rest of the sample. The supernatant is then removed by aspiration. The target molecule can be eluted off the beads with conventional elution methods and used in such applications as Western blot or mass spec analysis, or used in further downstream applications while still attached to the beads.

Automated Dynabeads separation helps increase throughput and reduces hands-on time
If you are working with several samples in parallel, the number of washing steps and the hands-on time increases proportionally with the number of samples. Pipetting and other manual handling tend to be less consistent than automation when working with many samples at a time. To better handle a medium- to high-throughput number of samples, reduce hands-on time, and secure high reproducibility, we have developed IP protocols for the KingFisher Flex and KingFisher Duo Prime instruments. The automated protocols replicate the manual protocols, obtaining equally high target protein yield and the same low non-specific binding and high reproducibility. It doesn’t matter if you are working with 10 or 96 samples, the IP protocol is less than 40 minutes regardless. Just load the reagents on the plates, push the “Start” button and by the time you have prepared for downstream analysis, the IP is done. Some optimization (e.g., incubation times) might be necessary depending on your antibody and the abundance and/or specificity of your target protein.

• Use the KingFisher Duo instrument for low to medium throughput (1–12 samples/run)
• Use the KingFisher Flex instrument for high throughput (12–96 samples/run)
• See automated protocols
• Watch a video about the KingFisher Flex instrument

Learn more about Dynabeads
• See immunoprecipitation selection guides, data, and references
• See magnets for Dynabeads separations
• Find Dynabeads products for other applications

OEM purchase
To purchase Dynabeads Sheep Anti-Rabbit IgG on an OEM basis, contact our Out-Licensing and OEM Sales department.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibody FragmentWhole Antibody
Certifications/ComplianceISO9001 and ISO13485
ClonalityPolyclonal
Concentration10 mg/mL
ContentsDynabeads M-280 Sheep Anti-Rabbit IgG is supplied in PBS, pH 7.4 w/0.1% BSA, 0.02% NaN3
DescriptionPolyclonal sheep anti-rabbit IgG covalently bound to Dynabeads
ColorBrown
For Use With (Application)Immunoprecipitation, Protein Purification
MaterialPolystyrene
Product TypeAntibody Coated Bead
For Use With (Equipment)KingFisher™ Sample Purification Systems, DynaMag™ Magnets
High-throughput CompatibilityHigh-throughput Compatible
Host SpeciesSheep
Isolation TechnologyMagnetic Beads
Ligand TypeAntibody
Preservative0.02% NaN3
Purification TargetProteins
Purity or Quality GradeResearch Grade
ReactivityBinds all rabbit IgG's
Shelf Life36 months from date of manufacture
Shipping ConditionAmbient temperature
Target SpeciesRabbit IgG's
UniformityMonosized 2.8 μm (CV <5%)
Volume (Metric)2 mL
Diameter (Metric)2.8 μm
Regulatory StatusFor Research Use Only
Unit SizeEach
Contents & Storage
Store at 2°C to 8°C.

Frequently asked questions (FAQs)

I am getting high background using Dynabeads M-280 Streptavidin magnetic beads. How can I prevent this from happening?

The background might be caused by nonspecific binding to the BSA on the bead surface. Alternatively, high background might be caused by nonspecific binding to streptavidin. Increasing either the pH or the salt concentration might help reduce the binding. Dynabeads M-270 Streptavidin magnetic beads might be a better alternative; these beads are not coated with BSA and are hydrophilic, as they are based upon carboxylic acid chemistry.

My Dynabeads magnetic beads are not pelleting well with the magnet. Do you have any suggestions for me?

Please review the following possibilities for why your Dynabeads magnetic beads are not pelleting:

- The solution is too viscous.
- The beads have formed aggregates because of protein-protein interaction.

Try these suggestions: - Increase separation time (leave tub on magnet for 2-5 minutes)
- Add DNase I to the lysate (~0.01 mg/mL)
- Increase the Tween 20 concentration to ~0.05% of the binding and/or washing buffer.
- Add up to 20 mM beta-merecaptoethanol to the binding and/or wash buffers.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

I have a long double-stranded DNA fragment I would like to isolate. What product do you recommend?

For biotin-labeled DNA that is less than 1 kb, we recommend you use Dynabeads M270 Streptavidin (Cat. No. 65305) and MyOne C1 magnetic beads (Cat. No. 65001). We recommend our Dynabeads KilobaseBINDER Kit (Cat. No. 60101), which is designed to immobilize long (>1 kb) double-stranded DNA molecules. The KilobaseBINDER reagent consists of M-280 Streptavidin-coupled Dynabeads magnetic beads along with a patented immobilization activator in the binding solution to bind to long, biotinylated DNA molecules for isolation. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/immobilisation-of-long-biotinylated-dna-fragments.html) for more information in regards to long biotinylated DNA fragment isolation.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

Can I use Dynabeads magnetic beads to isolate single-stranded DNA templates?

Yes, Dynabeads magnetic beads can be used to isolate single-stranded DNA. Streptavidin Dynabeads magnetic beads can be used to target biotinylated DNA fragments, followed by denaturation of the double-stranded DNA and removal of the non-biotinylated strand. The streptavidin-coupled Dynabeads magnetic beads will not inhibit any enzymatic activity. This enables further handling and manipulation of the bead-bound DNA directly on the solid phase. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/preparing-single-stranded-dna-templates.html) for more information in regards to single-stranded DNA capture.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

What is the magnetic susceptibility for Dynabeads magnetic beads?

Magnetic susceptibility is a measure of how quickly the beads will migrate to the magnet. This will depend on the iron content and the character of the iron oxide. The magnetic susceptibility given for the Dynabeads magnetic beads is the mass susceptibility, given either as cgs units/g or m^3/kg (the latter being an SI unit). For ferri- and ferromagnetic substances, the magnetic mass susceptibility is dependent upon the magnetic field strength (H), as the magnetization of such substances is not a linear function of H but approaches a saturation value with increasing field. For that reason, the magnetic mass susceptibility of the Dynabeads magnetic beads is determined by a standardized procedure under fixed conditions. The magnetic mass susceptibility given in our catalog is thus the SI unit. Conversion from Gaussian (cgs, emu) units into SI units for magnetic mass susceptibility is achieved by multiplying the Gaussian factor (emu/g or cgs/g) by 4 pi x 10^-3. The resulting unit is also called the rationalized magnetic mass susceptibility, which should be distinguished from the (SI) dimensionless magnetic susceptibility unit. In general, magnetic mass susceptibility is a measure of the force (Fz) influencing an object positioned in a nonhomogenous magnetic field. The magnetic mass susceptibility of the Dynabeads magnetic beads is measured by weighing a sample, and then subjecting the sample to a magnetic field of known strength. The weight (F1) is then measured, and compared to the weight of the sample when the magnetic field is turned off (F0). The susceptibility is then calculated as K x 10^-3 = [(F1-F0) x m x 0.335 x 10^6], where K is the mass susceptibility of the sample of mass m. The susceptibility is then converted to SI units.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

View all Dynabeads™ M-280 Sheep Anti-Rabbit IgG FAQs