Platinum™ Taq DNA Polymerase, High Fidelity
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Upgrade to Invitrogen™ Platinum™ SuperFi™ II DNA Polymerase: >300X higher fidelity than Taq, versatile and now enables universal primer annealing.
Platinum&trade; <i>Taq</i> DNA Polymerase, High Fidelity
Invitrogen™

Platinum™ Taq DNA Polymerase, High Fidelity

Platinum Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.
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Catalog NumberNo. of Reactions
11304029500 Reactions
11304011100 Reactions
113041025000 Reactions
Catalog number 11304029
Price (USD)
1,272.00
Each
Add to cart
No. of Reactions:
500 Reactions
Request bulk or custom format
Price (USD)
1,272.00
Each
Add to cart
Platinum Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum Taq DNA Polymerase and the proofreading enzyme. PCR specificity is improved with built-in Platinum hot-start technology.

Features of Platinum Taq DNA Polymerase, High Fidelity

  • Greater than six times higher fidelity than Taq DNA polymerase
  • Amplification of fragments up to 15 kb
  • Convenient room temperature reaction assembly
  • Increased specificity, and yield

Applications

  • Amplification of complex DNA templates
  • Amplification of viral and plasmid templates
  • RT-PCR
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceLow
PolymerasePlatinum Taq DNA Polymerase High Fidelity
Reaction SpeedStandard
Product TypeDNA Polymerase High Fidelity
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq)6X
Hot StartBuilt-In Hot Start
No. of Reactions500 Reactions
OverhangMixed
Reaction FormatSeparate Components
Size (Final Product)20 kb or less
Unit SizeEach
Contents & Storage
• Platinum Taq DNA Polymerase High Fidelity (1 x 100 μL at 5 U/μL)
• 10X High Fidelity Buffer (1 x 2.25 mL)
• 50 mM MgSO4 (1 x 1 mL)

Store at -10°C to -30°C.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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