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Dynabeads™ Human DC Enrichment Kit
Use this kit to enrich viable human dendritic cells (DC's) from PBMC by negative isolation. The kit depletes T cells,Read more
Catalog number 11308D
Price (USD)
1,218.00
Each
Price (USD)
1,218.00
Each
Use this kit to enrich viable human dendritic cells (DC's) from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes/ macrophages, granulocytes, NK cells and erythrocytes. Highly enriched DC's are bead and antibody-free, and can be used in any functional assay. The cells should be further sorted to obtain high purity of the specific DC sub-populations of choice.
• Obtain high recovery (80-100%) of viable DC's of all sup-populations.
• Substantial reduction in sorting time and volume compared to sorting only, thus saving money as well.
Methodology:
An antibody mix towards the non- dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched human DC's can be directly sorted into the sup-population of choice or used in any other application.
Starting samples:
PBMC
• Obtain high recovery (80-100%) of viable DC's of all sup-populations.
• Substantial reduction in sorting time and volume compared to sorting only, thus saving money as well.
Methodology:
An antibody mix towards the non- dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched human DC's can be directly sorted into the sup-population of choice or used in any other application.
Starting samples:
PBMC
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeDendritic Cells
Product TypeCell Enrichment Kit
Isolation TechnologyNegative Isolation
No. of CellsProcesses ∼2 x 109 cells total
Output Viability>95%
Product LineDYNAL™, Dynabeads™
Purity or Quality GradeResearch Grade
Quantity2 x 10 mL
Sample TypePBMC
Shipping ConditionRoom Temperature
Starting Material Cell No.1 x 107 PBMCs per isolation
Target SpeciesHuman
Unit SizeEach
Contents & Storage
The Kit Contains: 2 x 10 mL Depletion MyOne™ Streptavidin Dynabeads™ and 4 mL Antibody Mix containing an optimized mixture of biotinylated monoclonal antibodies towards CD3, CD14, CD16, CD19, CD56 and Glycophorin A.
Store at 2°C to 8°C.
Store at 2°C to 8°C.