SuperScript™ III Platinum™ One-Step qRT-PCR Kit
SuperScript™ III Platinum™ One-Step qRT-PCR Kit
SuperScript™ III Platinum™ One-Step qRT-PCR Kit
SuperScript™ III Platinum™ One-Step qRT-PCR Kit
SuperScript™ III Platinum™ One-Step qRT-PCR Kit
Invitrogen™

SuperScript™ III Platinum™ One-Step qRT-PCR Kit

The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenientRead more
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Catalog NumberNo. of Reactions
11732020100 Reactions
11732088500 Reactions
Catalog number 11732020
Price (USD)
592.00
Each
Add to cart
No. of Reactions:
100 Reactions
Price (USD)
592.00
Each
Add to cart
The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenient one-step format and consistent performance make it ideal for a number of gene expression applications, including infectious disease surveillance. The kit has been validated by several global public health organizations and has long been the choice of the US CDC for infectious disease surveillance.

Features of the SuperScript III Platinum One-Step qRT-PCR Kit include:
• SuperScript III RT for higher temperature cDNA synthesis when addressing difficult RNA secondary structure
• Platinum Taq DNA Polymerase with hot-start technology for improved specificity
• LUX fluorogenic primers and dual-labeled fluorogenic probes for effective detection performance
• Validated performance on multiple real-time instrument platforms, including rotor-based systems

SuperScript III Platinum One-Step qRT-PCR Kits come in multiple configurations:
SuperScript III Platinum One-Step qRT-PCR Kit (this page)
SuperScript III Platinum One-Step qRT-PCR Kit with ROX passive reference
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit with ROX passive reference

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
PCR Method1-step RT-qPCR
PolymeraseTaq DNA Polymerase
Reaction SpeedStandard
For Use With (Equipment)7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Product LinePlatinum™, SuperScript™
Product TypeOne-Step qRT-PCR Kit
Shipping ConditionDry Ice
Sufficient For100 Reactions
For Use With (Application)Gene Expression, Pathogen Detection, Virus Detection
No. of Reactions100 Reactions
Sample TypeRNA
Unit SizeEach
Contents & Storage
• 100 μl SuperScript™ III/Platinum™ Taq Mix
• 2 × 1.25 ml 2X Reaction Mix
• 1 ml Magnesium Sulfate (50 mM)
• 100 μl ROX Reference Dye (25 μM)

Store components at -20°C. ROX Reference Dye must be stored in the dark.

Frequently asked questions (FAQs)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.