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RNA UltraSense™ One-Step Quantitative RT-PCR System
The RNA UltraSense™ One-Step Quantitative RT-PCR System is specially designed for amplification and real-time quantification of ultra-low abundance transcripts andRead more
Catalog number 11732927
Price (USD)
590.00
Each
Price (USD)
590.00
Each
The RNA UltraSense™ One-Step Quantitative RT-PCR System is specially designed for amplification and real-time quantification of ultra-low abundance transcripts and RNA viruses. The optimized, ultra-concentrated system combines SuperScript™ III RT and Platinum™ Taq DNA Polymerase to provide greater priming specificity, higher product yields, and detection over a broad dynamic range. The high concentration formulation provides great flexibility with low concentration / high volume RNA samples, and improves performance with targets rich in secondary structure. As little as 25 pg/μl total RNA can be detected with a PCR efficiency of 97% (Figure 1). The RNA UltraSense™ System features:
• SuperScript™ III RT for higher temperature cDNA synthesis (up to 60°C) for greater success with RNA secondary structure
• Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity
• Optimized performance with either LUX™ Fluorogenic Primers or dual-labeled fluorogenic probes
• Proprietary, ultra-concentrated buffer for addition of large sample volumes (up to 70%) and success with RNA secondary structure
• Validated performance on multiple real-time instrument platforms
• One-step format for speed, convenience, and less reaction-to-reaction variability
Applications:
Real-time quantitative amplification of RNA, including:
• RNA viruses
• Low-abundance RNA transcripts
• Difficult-to-amplify RNA targets
• High-value RNA detection and research
• SuperScript™ III RT for higher temperature cDNA synthesis (up to 60°C) for greater success with RNA secondary structure
• Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity
• Optimized performance with either LUX™ Fluorogenic Primers or dual-labeled fluorogenic probes
• Proprietary, ultra-concentrated buffer for addition of large sample volumes (up to 70%) and success with RNA secondary structure
• Validated performance on multiple real-time instrument platforms
• One-step format for speed, convenience, and less reaction-to-reaction variability
Applications:
Real-time quantitative amplification of RNA, including:
• RNA viruses
• Low-abundance RNA transcripts
• Difficult-to-amplify RNA targets
• High-value RNA detection and research
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-qPCR
PolymeraseDNA Polymerase
Reaction SpeedStandard
Product LinePlatinum™, SuperScript™, UltraSense™
Product TypeOne-Step Quantitative RT-PCR System
Shipping ConditionDry Ice
Sufficient For100 Reactions
No. of Reactions100 Reactions
Sample TypeRNA
Unit SizeEach
Contents & Storage
The RNA UltraSense™ One-Step Quantitative RT-PCR System is supplied with the following components: 250 μl RNA UltraSense™ Enzyme Mix (includes SuperScript™ III RT, Platinum™ Taq DNA Polymerase, and RNaseOUT™ Ribonuclease Inhibitor); 2 x 1 ml RNA UltraSense™ 5X Reaction Mix; 300 μl 20X Bovine Serum Albumin (BSA), Ultrapure Molecular Biology Grade (5 mg/ml); 1 ml 50-mM magnesium sulfate (MgSO4 ); 100 μl ROX Reference Dye. Sufficient reagents are provided for 100 reactions. Store components at -20°C. Stability can be extended by storing at -80°C. ROX Reference Dye must be stored in the dark.