DMEM, high glucose, pyruvate
DMEM, high glucose, pyruvate
Gibco™

DMEM, high glucose, pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.Read more
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Catalog NumberQuantity
11995065500 mL
1199507310 x 500 mL
119950401000 mL
119950816 x 1000 mL
119951155 L
1199512310 L
Catalog number 11995065
Price (USD)
34.95
Each
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Quantity:
500 mL
Customize this product
Price (USD)
34.95
Each
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
WithWithout
• High Glucose• HEPES
• L-glutamine 
• Phenol Red 
• Sodium Pyruvate 


The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, 293, Cos-7, and PC-12
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Serum LevelStandard Serum Supplementation
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES
For Use With (Application)Mammalian Cell Culture
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity500 mL
Shipping ConditionAmbient
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

What is the difference between DMEM, high glucose, pyruvate Cat. No. 41966029 and Cat. No. 11995065?

Cat. No. 41966029 is manufactured in the Paisley, Scotland facility, while Cat. No. 11995065 is dual manufactured in Grand Island, New York and Paisley, Scotland. Although the formulation is said to be equivalent for molarities, the components may differ (e.g., it could be that one used L-Cystine 2HCl the other L-Cystine). This should not affect performance. Both facilities are FDA-registered and ISO 13485 certified.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

We do not provide stability data for the product once it is opened as it would depend on the usage and storage conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all DMEM, high glucose, pyruvate FAQs