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PureLink™ Pro 96 total RNA Purification Kit
The PureLink™ Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range ofRead more
Catalog number 12173011A
Price (USD)
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The PureLink™ Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.
Improved plate design
The PureLink™ Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.
Improved plate design
The PureLink™ Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologySilica Plate
Purification Time25 min. (After sample homogenization)
Elution Volume45–170 μL
Sample TypeBacteria, Cells, Plant, Tissue, Yeast
Final Product TypeTotal RNA
High-throughput CompatibilityHigh-throughput Compatible
For Use With (Equipment)Biomek FX, Robotic Liquid Handling Systems, Eppendorf epMotion TECAN Freedom Evo, TECAN Genesis
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity4 Plates
ScaleMini
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤109 cells
Cells: varies
Plant: ≤150 mg
Tissue: ≤100 mg
Yeast: ≤1.8 mL
Cells: varies
Plant: ≤150 mg
Tissue: ≤100 mg
Yeast: ≤1.8 mL
YieldUp to 33 μg
Unit SizeEach
Contents & Storage
• 150 mL PureLink Pro 96 Lysis Buffer; store at room temperature
• 250 mL PureLink Pro 96 Wash Buffer I; room temperature
• 250 mL PureLink Pro 96 Wash Buffer II; room temperature
• 75 mL PureLink Pro 96 RNase-free Water; room temperature
• 4 PureLink 96-well Total RNA Filter Plates; room temperature
• 4 PureLink Pro 96 Elution Plates; room temperature
• 250 mL PureLink Pro 96 Wash Buffer I; room temperature
• 250 mL PureLink Pro 96 Wash Buffer II; room temperature
• 75 mL PureLink Pro 96 RNase-free Water; room temperature
• 4 PureLink 96-well Total RNA Filter Plates; room temperature
• 4 PureLink Pro 96 Elution Plates; room temperature