PureLink™ DNase Set
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PureLink™ DNase Set
Invitrogen™

PureLink™ DNase Set

The PureLink™ DNase Set provides rapid and efficient removal of DNA from RNA that has been purified using PureLink™ RNARead more
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Catalog number 12185010
Price (USD)
151.00
Each
Add to cart
Price (USD)
151.00
Each
Add to cart
The PureLink™ DNase Set provides rapid and efficient removal of DNA from RNA that has been purified using PureLink™ RNA kits. The PureLink™ DNase Set is:

• RNase-free for use in RNA purification
• Optimized for on-column digestion
• Lyophilized for stability

Using the PureLink™ DNase Set
The RNase-free DNase set comes lyophilized and is optimized for on-column digestion of DNA using PureLink™ protocols. The degree of DNA digestion performed by PureLink™ DNase is optional for most PureLink™ RNA kits, due to the efficient removal of the majority of DNA by the PureLink™ technology; however, some downstream applications that are sensitive to small amounts of DNA may require further removal of DNA.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration10X
No. of Reactions50 Preps
Quantity50 Preps
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Contains:
• PureLink™ DNase (1500 U, lyophilized)
• 10X DNase I Reaction Buffer (1 mL)
• RNase-Free Water (1 mL)

Store at 4°C upon receipt. After reconstituted, PureLink™ DNase should be stored at -20°C.

Frequently asked questions (FAQs)

How can I inactivate DNase I?

Add of 1 µL of 25 mM EDTA solution to the reaction mixture in 10 µL reaction with 1 unit DNase I, Amplification Grade (or 1:1 molar ratio of Mg++ ions:EDTA) to chelate the Mg++ ions in the DNase I buffer. Heat for 10 min at 65 degrees C.

Note: It is vital that the EDTA be at least at 2 mM prior to heat-inactivation to avoid Mg-dependent RNA hydrolysis.

DNA-free and Turbo-free versions of DNase I can be inactivated with included DNase Inactivation Reagent.

Are your DNase I products RNase-free?

Most of our DNase I products are guaranteed free of RNase activity. However, please note that Cat. No. 18047-019 is not tested for RNAse and is recommended primarily for protein applications. The other products are suitable for removing DNA from both RNA and protein preparations, for nick translating DNA, and for generating random fragments of DNA. For more demanding RT-PCR applications, we recommend using DNAse I, Amplification Grade.