Oligofectamine™ Transfection Reagent
Oligofectamine™ Transfection Reagent
Invitrogen™

Oligofectamine™ Transfection Reagent

Oligofectamine™ Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA (siRNA) into eukaryotic cells. Oligofectamine™ TransfectionRead more
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Catalog number 12252011
Price (USD)
680.00
Each
Add to cart
Price (USD)
680.00
Each
Add to cart
Oligofectamine™ Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA (siRNA) into eukaryotic cells. Oligofectamine™ Transfection Reagent forms stable complexes with oligos, permitting efficient transfection into eukaryotic cells in a highly specific, yet nontoxic fashion. Oligofectamine™ Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO, HEK-293, NIH 3T3, and HeLa.

Using Oligofectamine™ Transfection Reagent
Oligofectamine™ Reagent is easy to use because it provides a simple and fast protocol. Just dilute Oligofectamine™ Reagent, mix with oligonucleotide, and add to your cells. Oligofectamine™ Reagent requires nanomolar quantities of antisense oligonucleotide, reducing the amount of valuable oligonucleotide needed by up to a thousand-fold. This makes it ideal for high-throughput applications. In addition, Oligofectamine™ Reagent has also been shown to work for siRNA transfections. We recommend this reagent when performing RNAi knockdown experiments in HeLa cells. Please visit RNAi Central for more information.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEstablished Cell Lines, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypeSynthetic siRNA
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
High-throughput CompatibilityHigh-throughput Compatible
Product LineOligofectamine™
Product TypeTransfection Reagent
Quantity1 mL
Serum CompatibleNo
Shipping ConditionWet Ice
Unit SizeEach
Contents & Storage
Contains one vial (1 ml) Oligofectamine™ Reagent. Store at 4°C. Do not freeze.

Frequently asked questions (FAQs)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

Is it necessary to use serum-free medium during lipid transfection?

It is not necessary to use serum-free medium during lipid transfection. However, it is critical to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum-containing medium. For optimal results with Lipofectin Transfection Reagent, we recommend performing transfection in medium without serum.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

View all Oligofectamine™ Transfection Reagent FAQs