AccuPrime™ Taq DNA Polymerase System
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AccuPrime&trade; <i>Taq</i> DNA Polymerase System
Invitrogen™

AccuPrime™ Taq DNA Polymerase System

The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.
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Catalog NumberNo. of Reactions
123390241000 Reactions
12339016200 Reactions
Catalog number 12339024
Price (USD)
2,040.00
Each
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No. of Reactions:
1000 Reactions
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Price (USD)
2,040.00
Each
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The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.

Platinum anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.

AccuPrime Taq DNA Polymerase features

  • Control mispriming at every cycle for single-band PCR products
  • Minimal reaction optimization and primer set redesign
  • Reaction assembly at room temperature

Applications

AccuPrime Taq DNA Polymerase can be used for a wide variety of applications, including:

  • Multiplex PCR
  • TOPO TA Cloning
  • Allele-specific amplifications
  • High-throughput screening

AccuPrime buffers

The 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime PCR Buffer II is designed for genomic DNA (200 bp–4 kb).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceLow
PCR Method1-step RT-qPCR, Multiplex qPCR
PolymeraseAccuPrime Taq DNA Polymerase
Reaction SpeedStandard
Product TypeDNA Polymerase System
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR
Concentration10X
Fidelity (vs. Taq)2X
Hot StartBuilt-In Hot Start
No. of Reactions1000 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Unit SizeEach
Contents & Storage
• AccuPrime Taq DNA Polymerase (500 μL, at 2 U/μL)
• 10X AccuPrime PCR Buffer I (2 x 1.25 mL)
• 10X AccuPrime PCR Buffer II (2 x 1.25 mL)
• 50 mM Magnesium Chloride (2 x 1.25 mL)

Store at -20°C.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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