293fectin™ Transfection Reagent
293fectin™ Transfection Reagent
Gibco™

293fectin™ Transfection Reagent

293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized forRead more
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Catalog NumberQuantity
123470191 mL
12347500
also known as 12347-500
15 mL
12347750
also known as 12347-750
10 x 15 mL
Catalog number 12347019
Price (USD)
680.00
Each
Add to cart
Quantity:
1 mL
Price (USD)
680.00
Each
Add to cart

293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (12338-018), and is intended for use with the FreeStyle™ 293 Expression System (K9000-01).

293fectin™ Transfection Reagent provides the following advantages:

  • Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells
  • Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required
  • Add 293fectin™ reagent complexes directly to cells in culture medium
  • No need to remove complexes or change or add medium following transfection
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEstablished Cell Lines
Sample TypePlasmid DNA
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product Line293fectin™
Product TypeTransfection Reagent
Quantity1 mL
Serum CompatibleYes
Unit SizeEach
Contents & Storage
Each unit contains one vial of reagent. Store at 4°C.
293fectin™ is guaranteed stable for 6 months when stored properly.

Frequently asked questions (FAQs)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

Is it necessary to use serum-free medium during lipid transfection?

It is not necessary to use serum-free medium during lipid transfection. However, it is critical to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum-containing medium. For optimal results with Lipofectin Transfection Reagent, we recommend performing transfection in medium without serum.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

View all 293fectin™ Transfection Reagent FAQs