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Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
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| Catalog Number | No. of Reactions |
|---|---|
| 12351010 | 100 Reactions |
| 12351050 | 500 Reactions |
| 12351250 | 5 x 500 Reactions |
Catalog number 12351010
Price (USD)
275.00
Each
No. of Reactions:
100 Reactions
Price (USD)
275.00
Each
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.
Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.
Benefits of Platinum SuperFi DNA Polymerase
- Exceptional >300X Taq fidelity
- High specificity and increased yields with Platinum hot-start technology
- Robust amplification of difficult targets including those of sub-optimal purity or high GC content
- Room temperature reaction setup and 24 hour bench top stability of pre-assembled reactions
- Amplification of samples with suboptimal purity
Applications
- High-fidelity PCR
- Cloning and sub-cloning
- Site-directed mutagenesis
- Amplification of GC-rich templates
- Template generation for sequencing
- High-throughput PCR
- Long PCR
Notes
- Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PolymerasePlatinum SuperFi DNA Polymerase
Reaction SpeedFast
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq)300X
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Size (Final Product)20 kb or less
Unit SizeEach
Contents & Storage
• Platinum SuperFi DNA Polymerase (50 μL at 2 U/μL)
• 5X SuperFi Buffer (2 x 1.25 mL)
• 5X SuperFi GC Enhancer (1.25 mL)
Store at –5°C to –30°C.
• 5X SuperFi Buffer (2 x 1.25 mL)
• 5X SuperFi GC Enhancer (1.25 mL)
Store at –5°C to –30°C.