Platinum™ SuperFi II DNA Polymerase
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Platinum™ SuperFi II DNA Polymerase
Invitrogen™

Platinum™ SuperFi II DNA Polymerase

Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.
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Catalog NumberNo. of Reactions
12361010100 Reactions
12361050500 Reactions
123612505 x 500 Reactions
Catalog number 12361010
Price (USD)
264.00
Each
Add to cart
No. of Reactions:
100 Reactions
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Price (USD)
264.00
Each
Add to cart
Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR. Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules.

Features of Platinum SuperFi II DNA Polymerase include:

  • Exceptional >300X Taq fidelity
  • Universal primer annealing at 60°C
  • Superior specificity, sensitivity, and yields
  • Robust amplification of difficult-to-amplify targets

Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction setup at room temperature and provides increased sensitivity and yield.

Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

Applications

  • High-fidelity PCR
  • Cloning and sub-cloning
  • Site-directed mutagenesis
  • Amplification of GC-rich templates
  • Template generation for sequencing
  • High-throughput PCR
  • Amplification of samples with suboptimal purity
  • Long PCR
Specifications
GC-Rich PCR PerformanceHigh
PolymerasePlatinum SuperFi II DNA Polymerase
Reaction SpeedFast
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq)>300X
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
OverhangBlunt
Reaction FormatStandalone
Size (Final Product)20 kb or less
Unit SizeEach
Contents & Storage
• Platinum SuperFi II DNA Polymerase (100 μL)
• 5X SuperFi II Buffer (1.25 mL)

Store at –20°C.

Frequently asked questions (FAQs)

What is the longest amplicon I can get using Platinum SuperFi II DNA Polymerase?

Platinum SuperFi II DNA Polymerase enables amplification of long targets (up to 20 kb).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

I'm having a hard time amplifying/cloning low copy cDNAs via PCR. Do you have any suggestions for me?

You may want to consider using our Platinum SuperFi II DNA Polymerase (Cat. No. 12361010) and try optimizing your PCR conditions if needed. The Platinum SuperFi II DNA Polymerase demonstrates a superior detection sensitivity with as little as 0.4 ng of genomic DNA. It is also a good idea to include a positive control reaction in parallel to your test PCR to ensure there are no issues with your reagents during handling or use over time. Please note that control templates may be ordered through our GeneArt Gene Synthesis (de novo sequence with 100% guarantee delivered in a vector) or Strings (up to 3 Kb, blunt-ended dsDNA pools, cloned into ZeroBlunt TOPO vector, convenient for multiple controls) services.

What are your recommendations when working with long amplicons using Platinum SuperFi II DNA Polymerase?

Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh, and intact) and fresh primer solutions. Reducing primer concentration to 0.2 µM may also improve the results.

Can I use Platinum SuperFi DNA Polymerase cycling protocol and primer annealing temperature with Platinum SuperFi II DNA Polymerase?

Yes, Platinum SuperFi II DNA Polymerase works at both universal 60 degrees C annealing temperature and at annealing temperatures calculated with a Tm calculator.

Can I perform TA cloning with Platinum SuperFi II DNA Polymerase?

Platinum SuperFi II DNA Polymerase produces blunt-ended PCR products that can be cloned directly into blunt-ended cloning vectors. TA cloning is also possible if 3' dA-overhangs are added after PCR. We recommend purifying the PCR products of Platinum SuperFi DNA Polymerase before adding the overhangs. The procedure for adding 3' dA-overhangs (TA cloning) includes the following steps:
- Purify the PCR product (e.g., with a PCR purification kit or phenol extraction/DNA precipitation). Before adding the overhangs, PCR product must be purified, as the strong proofreading activity of any remaining Platinum SuperFi DNA Polymerase will degrade the added 3' dA-overhangs.
- Perform 3' dA addition with a Taq DNA polymerase.
Reaction components:
Purified PCR product
0.2 mM dATP
1x Taq Buffer
1 U Taq DNA Polymerase
Incubate the reaction for 20 min at 72 degrees C.
- Proceed to TA cloning. For optimal ligation efficiency, we recommend using fresh PCR products, since 3' dA-overhangs can be lost during storage

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