Platinum™ SuperFi II PCR Master Mixes
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Platinum™ SuperFi II PCR Master Mixes
Invitrogen™

Platinum™ SuperFi II PCR Master Mixes

Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
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Catalog NumberColorNo. of Reactions
12368010Colorless100 Reactions
12369010Green100 Reactions
12369050Green500 Reactions
12369250Green5 x 500 Reactions
12368050Colorless500 Reactions
12368250Colorless5 x 500 Reactions
Catalog number 12368010
Price (USD)
355.00
Each
Add to cart
Color:
Colorless
No. of Reactions:
100 Reactions
Request bulk or custom format
Price (USD)
355.00
Each
Add to cart
Invitrogen Platinum SuperFi II Green PCR Master Mix (2X) contains a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup, as well as two tracking dyes for direct loading of PCR products on gels. Platinum SuperFi II DNA Polymerase (Cat. No. 12361010) is a proofreading DNA polymerase that combines superior fidelity with innovative SuperFi II Buffer, enabling universal primer annealing for the highest success in PCR. It is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Features of Platinum SuperFi II PCR Master Mix include:

  • Exceptional >300X Taq fidelity
  • Universal primer annealing at 60°C
  • Superior specificity, sensitivity, and yields
  • Robust amplification of difficult-to-amplify targets (e.g., those with suboptimal purity, ˃65% GC content, long PCR requirement)

Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction set up at room temperature and provides increased sensitivity and yield.

Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

Applications of Platinum SuperFi II DNA Polymerase:

  • High-fidelity PCR
  • Cloning and sub-cloning
  • Site-directed mutagenesis
  • Amplification of GC-rich templates
  • Template generation for sequencing
  • High-throughput PCR
  • Amplification of samples with suboptimal purity
  • Long PCR
  • Fast PCR

Platinum SuperFi II PCR Master Mix (12368010) is also available, which is the same master mix without the tracking dyes.

Specifications
GC-Rich PCR PerformanceHigh
PolymerasePlatinum SuperFi II DNA Polymerase
Reaction SpeedFast
Product TypePCR Master Mix
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Concentration2X
Fidelity (vs. Taq)>300X
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
OverhangBlunt
Reaction FormatSuperMix or Master Mix
Size (Final Product)20 kb or less
ColorColorless
Unit SizeEach
Contents & Storage
• Platinum SuperFi II PCR Master Mix (2 x 1.25 mL)
• Nuclease-free water (2 x 1.25 mL)

Store at –20°C.

Frequently asked questions (FAQs)

What are your recommendations when working with long amplicons using Platinum SuperFi II DNA Polymerase?

Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh, and intact) and fresh primer solutions. Reducing primer concentration to 0.2 µM may also improve the results.

Can I use Platinum SuperFi DNA Polymerase cycling protocol and primer annealing temperature with Platinum SuperFi II DNA Polymerase?

Yes, Platinum SuperFi II DNA Polymerase works at both universal 60 degrees C annealing temperature and at annealing temperatures calculated with a Tm calculator.

Can I perform TA cloning with Platinum SuperFi II DNA Polymerase?

Platinum SuperFi II DNA Polymerase produces blunt-ended PCR products that can be cloned directly into blunt-ended cloning vectors. TA cloning is also possible if 3' dA-overhangs are added after PCR. We recommend purifying the PCR products of Platinum SuperFi DNA Polymerase before adding the overhangs. The procedure for adding 3' dA-overhangs (TA cloning) includes the following steps:
- Purify the PCR product (e.g., with a PCR purification kit or phenol extraction/DNA precipitation). Before adding the overhangs, PCR product must be purified, as the strong proofreading activity of any remaining Platinum SuperFi DNA Polymerase will degrade the added 3' dA-overhangs.
- Perform 3' dA addition with a Taq DNA polymerase.
Reaction components:
Purified PCR product
0.2 mM dATP
1x Taq Buffer
1 U Taq DNA Polymerase
Incubate the reaction for 20 min at 72 degrees C.
- Proceed to TA cloning. For optimal ligation efficiency, we recommend using fresh PCR products, since 3' dA-overhangs can be lost during storage

Can I perform restriction digestion in the Platinum SuperFi II reaction buffer?

Platinum SuperFi II reaction buffer is compatible with restriction digestion directly after PCR. Note that Platinum SuperFi II DNA Polymerase is not inactivated during PCR, thus purification of PCR products before restriction digestion is recommended if intact 5'- or 3'- overhangs are needed for cloning.

Does the green tracking dye in Platinum SuperFi II Green PCR Master Mix interfere with downstream applications?

The green tracking dye (which consists of a blue and a yellow dye) is compatible with downstream applications such as fluorescent automated DNA sequencing, ligation, and restriction digestion. For applications that require analysis of PCR products by absorbance or fluorescence excitation, we recommend using colorless versions of the products (Platinum SuperFi II DNA Polymerase or Platinum SuperFi II PCR Master Mix) or purifying the PCR products prior to analysis.

View all Platinum™ SuperFi II PCR Master Mixes FAQs