Platinum™ PCR SuperMix High Fidelity
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Upgrade to Invitrogen™ Platinum™ SuperFi™ II DNA Polymerase: >300X higher fidelity than Taq, versatile and now enables universal primer annealing.
Platinum™ PCR SuperMix High Fidelity
Invitrogen™

Platinum™ PCR SuperMix High Fidelity

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
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Catalog NumberNo. of Reactions
12532016100 Reactions
125320245000 Reactions
Catalog number 12532016
Price (USD)
403.00
Each
Add to cart
No. of Reactions:
100 Reactions
Request bulk or custom format
Price (USD)
403.00
Each
Add to cart
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Designed for high-specificity, high-fidelity DNA amplification, Platinum PCR SuperMix reduces reaction set-up time and only requires the addition of template and primer.

High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic hot-start PCR.

Benefits of Platinum PCR SuperMix, High Fidelity

  • Six-times higher fidelity than Taq DNA polymerase
  • Amplifies fragments up to 15 kb
  • Minimizes PCR optimization
  • Room-temperature reaction assembly

Applications

  • High-fidelity PCR
  • Cloning
  • Mutagenesis

Notes

  • Platinum PCR SuperMix, High Fidelity, produces products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs.
  • Hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceLow
PolymerasePlatinum Taq DNA Polymerase High Fidelity
Reaction SpeedStandard
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Concentration1.1X
Fidelity (vs. Taq)6X
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
OverhangMixed
Reaction FormatSuperMix or Master Mix
Size (Final Product)15 kb or less
Unit SizeEach
Contents & Storage
Platinum PCR SuperMix High Fidelity (4 x 1.125 mL)

Store at -20°C in a non-frost-free freezer.