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Gibco™
TrypLE™ Select Enzyme (1X), no phenol red
Gibco TrypLE Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells.
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| Catalog Number | Quantity |
|---|---|
| 12563029 | 500 mL |
| 12563011 | 100 mL |
Catalog number 12563029
Price (USD)
173.00
Each
Quantity:
500 mL
Price (USD)
173.00
Each
Gibco TrypLE Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE formulations for a range of cell culture applications.
Compared to trypsin and other dissociation reagents, TrypLE Select is:
• Gentle on cells—maintains cell health for reproducible results
• Room-temperature stable—convenient to use
• Easy to use—substitutes directly into existing protocols with no inactivation required
• Animal origin free (AOF)—pure, consistent, and effective
This TrypLE Select is modified as follows:
With
• EDTA
Without
• Phenol Red
Gentle on cells
TrypLE Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room-temperature stable
TrypLE Select is room-temperature stable and ready to use. TrypLE Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Select, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE Select is free of animal-derived components and is formulated on dedicated animal origin-free equipment.
Product Intended Use
Customers using TrypLE Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
TrypLE Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
Specifications
ChelatorsEDTA
Cell TypeMammalian Cells
ClassificationAnimal Origin-free
Concentration1 X
Culture TypeAdherent Cell Culture
FormLiquid
Product TypeCell Culture Dissociation Reagent
Shelf Life24 Months
Without AdditivesNo Phenol Red
Endotoxin LevelLow
FormatBottle(s)
Green FeaturesEnergy efficient, Sustainable packaging
Osmolality280 - 300 mOsm/kg
pH7 to 7.4
Product LineGibco™
Quantity500 mL
RemarksRoom Temperature Stable
Shipping ConditionRoom Temperature
SourceAnimal origin-free
Tested ForIn vitro Bioassay
Unit SizeEach
Contents & Storage
Storage conditions: 15–30°C, protect from light.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.