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SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
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| Catalog Number | Includes | No. of Reactions |
|---|---|---|
| 12594025 | Kit only | 25 Reactions |
| 12594100 | Kit only | 100 Reactions |
| 12595025 | Kit with ezDNase | 25 Reactions |
| 12595100 | Kit with ezDNase | 100 Reactions |
Catalog number 12594025
Price (USD)
400.00
Each
Includes:
Kit only
No. of Reactions:
25 Reactions
Price (USD)
400.00
Each
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.
Features of the SuperScript IV One-Step RT-PCR System include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
• Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only)
SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.
The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodGel Electrophoresis
FormatKit
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
PolymerasePlatinum SuperFi
Reaction Speed10 min.
Technique1-step RT-PCR
Optimal Reaction Temperature45°C to 60°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Final Product TypePCR Amplified cDNA
Hot StartBuilt-In Hot Start
No. of Reactions25 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 14 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Contents & Storage
• SuperScript IV RT Mix (12.5 μL)
• 2X Platinum SuperFi RT-PCR Master Mix (625 μL)
• Nuclease-free water (1.25 mL)
Store at –20°C.