Lipofectamine™ RNAiMAX Transfection Reagent
See the new Lipofectamine® RNAiMAX Transfection Reagent protocol—now optimized for efficiency, viability and reproducibility
Lipofectamine™ RNAiMAX Transfection Reagent
Lipofectamine™ RNAiMAX Transfection Reagent
Lipofectamine™ RNAiMAX Transfection Reagent
Invitrogen™

Lipofectamine™ RNAiMAX Transfection Reagent

Green features
Lipofectamine™ RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdownRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
137780750.75 mL
137781501.5 mL
137781000.1 mL
137780300.3 mL
1377850015 mL
Catalog number 13778075
Price (USD)
630.00
Each
Add to cart
Quantity:
0.75 mL
Price (USD)
630.00
Each
Add to cart
Lipofectamine™ RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine™ RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types.

With Lipofectamine™ RNAiMAX Transfection Reagent you will get:

• Superior transfection efficiency requiring lower RNAi concentrations, leading to more effective gene knockdown with minimal nonspecific effects
• Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent
• Superior transfection efficiencies for miRNA antagonists and mimics
• Compatibility with a broad range of cell types, providing the most versatile approach to all of your gene silencing experiments
• A simple and rapid protocol for consistent and reproducible results

High knockdown in a wide range of cells
Lipofectamine™ RNAiMAX Transfection Reagent transfects a wide range of cell types (see figure). For gene silencing, Lipofectamine™ RNAiMAX Transfection Reagent's high-efficiency transfections lead to the high levels of gene knockdown needed to achieve convincing results.

Simple, high-throughput–ready transfections
Simply mix Lipofectamine™ RNAiMAX Transfection Reagent with siRNA, add to your cells, incubate, and measure gene knockdown. The simplicity and speed combined with high transfection efficiency make Lipofectamine™ RNAiMAX Transfection Reagent ideal for high-throughput siRNA transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.

Find an optimized Lipofectamine™ RNAiMAX transfection protocol for your cell line.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypeSynthetic siRNA, siRNA, miRNA
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
High-throughput CompatibilityHigh-throughput Compatible
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity0.75 mL
Serum CompatibleYes
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Unit SizeEach
Contents & Storage
Contains one vial (0.75 ml) Lipofectamine™ RNAiMAX Transfection Reagent. Store at 2–8°C. Do not freeze.

Frequently asked questions (FAQs)

I'm seeing cell death after transfection using Lipofectamine RNAiMAX Reagent. What should I do?

Remove antibiotics from the medium during transfection, as this can cause stress leading to cell death. Try adjusting both lipid and siRNA quantities. Use cells with lower passage number for better viability. Consider assaying for microbes such as Mycoplasma that are not detected easily in cell culture.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I accidentally kept my Lipofectamine RNAiMAX Reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I accidentally froze my Lipofectamine RNAiMAX Reagent. Can I still use it?

Freezing can alter the integrity and composition of the lipid particles. The performance of the reagent may be affected and it may only work for the first week or so. It is safer to use a new vial.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What positive transfection control can I use with Lipofectamine RNAiMAX?

BLOCK-iT Alexa Fluor Red Fluorescent Control (Cat. No. 14750100) can be used as an indicator of transfection efficiency of siRNA. This dye can be detected using standard filter sets designed for Alexa Fluor 555, Cy3, DsRed, Texas Red, or Rhodamine Red fluorophores.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.