Platinum™ II Hot-Start PCR Master Mixes (2X)
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Platinum™ II Hot-Start PCR Master Mixes (2X)
Invitrogen™

Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
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Catalog NumberNo. of ReactionsColor
1400001250 reactionsColorless
1400101250 ReactionsGreen
14001013200 ReactionsGreen
140010141000 ReactionsGreen
140000141000 reactionsColorless
14000013200 ReactionsColorless
Catalog number 14000012
Price (USD)
103.00
Each
Add to cart
No. of Reactions:
50 reactions
Color:
Colorless
Request bulk or custom format
Price (USD)
103.00
Each
Add to cart
Invitrogen Platinum II Hot-Start PCR Master Mix (2X), available in colorless and green formats, offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase and leading hot-start technology. This master mix is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Platinum II Hot-Start Green PCR Master Mix also includes two tracking dyes for direct loading of PCR products on gels.

Features

  • Innovative buffer enables universal annealing temperature by isostabilizing primer-template duplex structures
  • Engineered Taq DNA polymerase confers fast cycling and resistance to common inhibitors
  • Hot-start technology enables superior specificity, sensitivity, and yields and allows for room temperature reaction setup
  • Green PCR buffer reduces pipetting errors with direct gel loading

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • Amplification and improved yields of GC-rich targets
  • RT-PCR
  • Genotyping
  • High-throughput PCR

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and delivers PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Hot-Start PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Notes

  • For applications that require PCR product analysis by absorbance or fluorescence excitation, colorless format is recommended.
For Research Use Only.
Specifications
FormatTube
GC-Rich PCR PerformanceHigh
PolymerasePlatinum II Taq Hot-Start DNA Polymerase
Reaction SpeedFast or Standard
Product TypeHot Start PCR Master Mix
Purity or Quality GradeHPLC
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR
Concentration2X
Fidelity (vs. Taq)1X
Hot StartBuilt-In Hot Start
No. of Reactions50 reactions
Overhang3'-A
Reaction FormatSuperMix or Master Mix
Size (Final Product)5 kb or less
Starting MaterialDNA
ColorColorless
Unit SizeEach
Contents & Storage
• Platinum II PCR Master Mix (2X), 1.25 mL
• Platinum GC Enhancer, 1.25 mL
• Nuclease-free water, 1.25 mL

Store at -20°C in a non-frost-free freezer.

Frequently asked questions (FAQs)

What is the storage temperature of Platinum II Taq Hot-Start DNA Polymerase products?

Platinum II Taq Hot-Start DNA Polymerase products can be stored at 4 degrees C for up to 3 months. For longer storage, we recommend storing all components at -20 degrees C.

Is the PCR product from reactions performed with Platinum II Hot-Start PCR Master Mix and Platinum II Hot-Start Green PCR Master Mix compatible with E-Gel agarose gels?

Electrophoretic separation on E-Gel agarose gels depends on the salt concentration in the analyzed sample. For optimal separation, we recommend diluting PCR reactions performed with colorless and green Platinum II PCR Master Mixes 2- to 20-fold, prior to running on E-Gel agarose gels. The dyes in the Platinum II Hot-Start Green PCR Master Mix do not interfere with fragment separation on E-Gel agarose gels.

Do the dyes in Platinum II Green PCR Buffer interfere with PCR performance of Platinum II Taq Hot-Start DNA Polymerase?

No. The tracing dyes (a blue and a yellow dye) in Platinum II Green PCR Buffer do not interfere with PCR performance and do not change any enzyme features.

Can Platinum II Taq Hot-Start DNA Polymerase be used in master mixes for qPCR?

Yes, Platinum II Taq Hot-Start DNA Polymerase can be used in qPCR master mixes for target detection and quantification in a real-time PCR instrument using probes or SYBR Green dye.

Can I keep my PCR reactions with Platinum II Taq Hot-Start DNA Polymerase at room temperature before the cycling starts?

Yes. Due to stable antibody-mediated hot-start technology, Platinum II Taq Hot-Start DNA Polymerase is highly stable. The premixed reactions for PCR can be incubated at room temperature for up to 24 hr before loading in the thermal cycler, without any loss of amplification specificity.

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