BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control
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Invitrogen™

BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control

The BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control is an ideal indicator of lipid-mediated transfection efficiency for RNAi experiments, particularly thoseRead more
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Catalog number 14750100
Price (USD)
470.00
Each
Add to cart
Price (USD)
470.00
Each
Add to cart
The BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control is an ideal indicator of lipid-mediated transfection efficiency for RNAi experiments, particularly those using the Lipofectamine™ RNAiMAX Transfection Reagent. The sequence of the BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control is not homologous to any known gene, preventing induction of nonspecific cellular events caused by introduction of the duplex into cells. The BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control provides:
• A strong, easily detectable, and photostable Alexa Fluor™ 555* signal indicating transfection efficiency
• Chemical modifications for a clearer, more persistent cellular signal than with labeled siRNA
• Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules *This dye can be detected using standard filter sets designed for Alexa Fluor™ 555, Cy™3, DsRed, Texas Red™, or rhodamine fluorophores.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Control TypeTransfection Control
Detection MethodFluorescence
Sample TypeRNA
For Use With (Application)RNA Transfection
Label or DyeAlexa Fluor 555
Product LineAlexa Fluor™, BLOCK-iT™
Product TypeRed fluorescent transfection control
Quantity2 x 125 μL
RNAi TypesiRNA
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
The BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control is provided at a concentration of 20 μM. Store at -20°C. Guaranteed stable for 12 months when properly stored.

Frequently asked questions (FAQs)

What are the positive and negative controls I should use with my Stealth RNAi siRNA experiment?

We offer several negative and positive controls for Stealth siRNA:

For Stealth Positive Control:
- Stealth RNAi siRNA GAPDH Positive Control (human) (Cat. No. 12935140, 250 µL)
- Stealth RNAi siRNA beta-Lactamase Reporter Control (Cat. No. 12935148, 250 µL)
- Stealth RNAi siRNA GFP Reporter Control (Cat. No. 12935145, 250 µL)
- Stealth RNAi siRNA Luciferase Reporter Control (Cat. No. 12935146, 250 µL)

For Stealth Negative Control:
Hi (55-65% GC), Med (45-55% GC), or Low (35-45% GC) Duplexes are offered:

- Stealth RNAi siRNA Negative Control Hi GC (Cat. No. 12935400, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #2 (Cat. No. 12935114, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935115, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex (Cat. No. 12935300, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Cat. No. 12935112, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935113, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex (Cat. No. 12935200, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #2 (Cat. No. 12935110, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #3 (Cat. No. 12935111, 250 µL)
- Stealth RNAi siRNA Negative Control Kit (Cat. No. 12935100, contains one of each GC content level negative control, 250 µL each at 20 µM)
- For a scrambled negative control, you could also scramble your designed siRNA or Stealth RNAi sequence using the BLOCK-iT RNAi Designer.

You can also use our BLOCK-iT AlexaFluor Red Fluorescent Control (Cat. No. 14750100) or BLOCK-iT Fluorescent Oligo (Cat. No. 2013, 13750062) as a control for the transfection.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Can I use the BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100)?

  • We do not recommend using BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100). BLOCK-iT Alexa Fluor Red Fluorescent Control (Cat. No. 14750100) should be used to assess transfection efficiency with Lipofectamine RNAiMAX Reagent.
  • BLOCK-iT Fluorescent Oligo, for lipid transfection is optimized for use with Lipofectamine 2000 Transfection Reagent and is not recommended for Lipofectamine RNAiMAX Reagent.


    • Find additional tips, troubleshooting help, and resources within our RNAi Support Center