Versene Solution
Versene Solution
Gibco™

Versene Solution

Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco Versene Solution (0.48 mM) isRead more
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Catalog number 15040066
Price (USD)
16.04
Each
Add to cart
Price (USD)
16.04
Each
Add to cart
Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na4) per liter of Phosphate Buffered Saline (PBS).

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco Versene Solution at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ChelatorsEDTA
ClassificationAnimal Origin-free
Product TypeCell Culture Dissociation Reagent
Shelf Life18 Months
SterilitySterile-filtered
With AdditivesEDTA
Green FeaturesSustainable packaging
Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485
Quantity100 mL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 2 to 8°C
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture

Frequently asked questions (FAQs)

I currently use mTeSR1. How difficult would it be to adapt my cells to the StemFlex Medium system?

This transition is very straightforward. We recommend at least a 2-passage transition into the StemFlex Medium system. Briefly, if you have cryopreserved cells previously cultured in the mTeSR1/Matrigel system, we recommend thawing the cells back into mTeSR1/Matrigel until fully recovered. Upon reaching ~70-85% confluency, passage using Versene solution and seed directly into the StemFlex Medium system.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Can I use EDTA or versene for passaging of stem cells?

Yes, you may use EDTA or versene. However, we do not recommend using dispase or collagenase as it can lead to differentiation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to add a ROCK inhibitor during passaging when culturing in Gibco Essential 8 Flex Medium?

As with many other PSC media, ROCK inhibitor is not required with Essential 8 Flex Medium when Gibco Versene Solution is used to passage PSCs as clusters. If cells are plated as singlets, we suggest that Gibco RevitaCell Supplement (Cat. No A2644501) or another ROCK inhibitor be used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is Gibco Versene Solution still the recommended dissociation reagent with Gibco Essential 8 Flex Medium?

Both Versene Solution (Cat. No. 15040066) and enzyme-mediated dissociation have been shown to be compatible passaging methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.