Pierce™ Glutathione Coated Plates
Pierce™ Glutathione Coated Plates
Pierce™ Glutathione Coated Plates
Pierce™ Glutathione Coated Plates
Thermo Scientific™

Pierce™ Glutathione Coated Plates

Thermo Scientific™ Pierce Glutathione Coated Plates are standard 96-well microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.
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Catalog NumberDescriptionDetection Method
15140Clear, 8-well StripColorimetric
15340Black, 96-wellFluorescence
15240White, 96-wellChemiluminescence
Catalog number 15140
Price (USD)
268.00
Each
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Description:
Clear, 8-well Strip
Detection Method:
Colorimetric
Request bulk or custom format
Price (USD)
268.00
Each
Add to cart
Pierce Glutathione Coated Plates are coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Plates can also be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein. As the plates are pre-blocked, initial purification of the cell lysates is not necessary.
Thermo Scientific Pierce Glutathione Coated Plates are microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.

Features of Glutathione Coated Plates include:
Selective—specifically binds fusion proteins containing glutathione S-transferase
Capacity—binds approx. 10 ng GST protein per well
Versatile—ready-to-bind plate format is useful for protein interaction studies
Compatible—detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
Ready to use—Pre-blocked with SuperBlock Blocking Buffer to reduce nonspecific binding

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Binding PropertyStandard Capacity, Affinity
DescriptionClear, 8-well Strip
Detection MethodColorimetric
Packaging5 Plates
Product LinePierce
ColorClear
Product TypeMicroplate
MaterialPolystyrene
No. of Wells96
Surface TreatmentGlutathione
Plate BlockingSuperBlock™ Buffer
Unit SizeEach
Contents & Storage
Store at 4°C in unopened pouches. Once opened, store unused plates in a resealable bag with desiccant at 4°C.

Frequently asked questions (FAQs)

How does Penicillin work?

Penicillin interferes with the final stages of bacterial cell wall synthesis.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the detection limit of proteins with Pierce Glutathione Coated Plates?

The background is extremely low when using the Pierce Glutathione Coated Plates. This results in a high signal-to-noise ratio and a low detection limit which in general means that the detection limit (OD 0.5) is 3 ng per well (100 µL).

How long are Pierce Glutathione Coated Plates stable?

The Pierce Glutathione Coated Plates are extremely stable over long periods of time, if stored at room temperature (20-25 degrees C). We guarantee 12 mths of shelf life in unopened foil package after the date of shipment.

Can I determine the concentration of an unknown GST-tagged protein?

To determine the approximate concentration of a GST-tagged protein, a standard curve should be generated using purified GST added in different concentrations to the wells of the plates. Please note that it is only the approximately concentration that can be determined since not 100% of the GST-fusion proteins will be bound due to denaturing or steric hindrance to GSH.

Can I whirl-mix solutions containing GST proteins?

No. Do not whirl-mix a solution containing GST-tagged proteins. The CV will increase due to slight degradation of the GST protein.