Gibco™ Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. This solution contains 10,000 units/mL of penicillin, 10,000 μg/mL ofRead more
Have Questions?
Change view
Catalog Number
Quantity
15240062
100 mL
15240096
20 mL
15240112
20 x 100 mL
3 Options
Catalog number 15240062
Price (USD)
53.25
Each
Add to cart
Quantity:
100 mL
Price (USD)
53.25
Each
Add to cart
Gibco™ Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. This solution contains 10,000 units/mL of penicillin, 10,000 μg/mL of streptomycin, and 25 μg/mL of Gibco Amphotericin B. The antibiotics penicillin and streptomycin prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Amphotericin B prevents fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeast.
Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Amphotericin B is an antifungal agent that prevents the growth of fungi and yeast by causing an increase in fungal plasma membrane permeability.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration100 X
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeAntibiotic-Antimycotic
Shelf Life12 Months
SterilitySterile-filtered
Quantity100 mL
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Storage conditions: -5°C to -20°C Shipping conditions: Dry ice Shelf life: 12 months from date of manufacture
Frequently asked questions (FAQs)
Can I use pen/strep or other antibiotics for episomal reprogramming of cells?
The use of antibiotics is not recommended during episomal reprogramming, especially Fungizone antimycotic. If antibiotics must be used during the reprogramming process, pen/strep could be used. For established iPSCs (after reprogramming is complete), either antibiotic/antimycotic or pen/strep should be fine.
Can I use antibiotics such as Pen-Strep or Antibiotic-Antimycotic (100X) when culturing Sf9 insect cells?
Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.