Amphotericin B
Amphotericin B
Gibco™

Amphotericin B

Amphotericin B is the generic version of Fungizone. 'Fungizone' is a trademark of E.R. Squibb & Sons, LLC. Amphotericin BRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
1529001820 mL
1529002650 mL
Catalog number 15290018
Price (USD)
34.85
Each
Add to cart
Quantity:
20 mL
Price (USD)
34.85
Each
Add to cart
Amphotericin B is the generic version of Fungizone. 'Fungizone' is a trademark of E.R. Squibb & Sons, LLC. Amphotericin B is an antifungal, produced by Streptomyces nodosus. It prevents the growth of fungi by causing an increase in fungal plasma membrane permeability. It actively binds to sterols and leads to the formation of pores. It is used to prevent the contamination of cell cultures by yeast and multicellular fungi. Gibco Amphotericin B contains 250 μg of amphotericin B and 205 μg of sodium deoxycholate per mL of distilled water. The recommended working concentration ranges from 0.25 to 2.50 μg/mL.

Dual-site cGMP manufacturing
For supply chain continuity, we manufacture Gibco Amphotericin B at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration0.25 to 2.5 μg/mL
FormLiquid
Product TypeAntifungal
Shelf Life12 Months
SterilitySterile-filtered
For Use With (Application)Prevention of Cell Culture Contamination
Product LineFungizone™
Quantity20 mL
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Storage conditions: -5°C to -20°C
Shipping conditions: Frozen on dry ice
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.