UltraPure™ Dithiothreitol
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Invitrogen™

UltraPure™ Dithiothreitol

UltraPure™ Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymesRead more
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Catalog number 15508013
Price (USD)
187.00
Each
Add to cart
Price (USD)
187.00
Each
Add to cart
UltraPure™ Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups. Higher concentrations of DTT are used to cleave disulfide linkages in polypeptides and to facilitate protein denaturation by detergents or chaotropic agents.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Protein Electrophoresis & Western Blotting
Product LineUltraPure™
Quantity5 g
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Unit SizeEach
Contents & Storage
Store in freezer (-5° to -30°C).

Frequently asked questions (FAQs)

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How do I prepare a 1 M DTT solution from powder and how should I store the solution?

Preparing 1 M DTT:
1) Dissolve 3.09 g DTT in 20 mL distilled H2O.
2) Dispense into 1 mL aliquots.
3) Store the aliquots at -20 degrees C.
Note: Upon thawing the vial, there may be some precipitation that can be re-dissolved by warming and mixing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.