X-Gal
X-Gal
Invitrogen™

X-Gal

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.
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Catalog NumberQuantity
155200181 g
15520034100 mg
Catalog number 15520018
Price (USD)
354.00
Each
Add to cart
Quantity:
1 g
Price (USD)
354.00
Each
Add to cart
X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing X-Gal. X-Gal is supplied as white crystals and is soluble in dimethylformamide.

Primary applications

  • Blue-white screening—detect functional β-galactosidase (reporter gene expression)
  • Histochemical detection of β-galactosidase activity in fixed tissues
  • X-gal overlay assay

Find the media format that fits your needs

We offer many ready-to-use media formulations, such as LB Broth (10855001), Terrific Broth (A1374301) or S.O.C recovery medium (15544034).

We also offer IPTG (15529019) for blue-white screening.

Explore other media formats for bacterial growth.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or Material5-bromo-4-chloro-3-indolyl-β-D-galactoside
Detection MethodColorimetric
DescriptionX-Gal, Cell-Permeant
FormatBottle
For Use With (Application)Immunoblotting
Product TypeX-Gal Substrate
Quantity1 g
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
FormPowder
Unit SizeEach
Contents & Storage
• X-Gal, powder, 1 g