UltraPure™ Ethidium Bromide, 10 mg/mL
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UltraPure™ Ethidium Bromide, 10 mg/mL
Invitrogen™

UltraPure™ Ethidium Bromide, 10 mg/mL

UltraPure™ Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and Caesium chloride (CsCl)Read more
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Catalog number 15585011
Price (USD)
112.00
Each
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Price (USD)
112.00
Each
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UltraPure™ Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and Caesium chloride (CsCl) gradients. It is available as an aqueous solution containing 10 mg/ml UltraPure™ Ethidium Bromide and high-purity water.
• Sensitive: Detect as little as little as 1 ng of nucleic acids in agarose gels
• Versatile: Detect DNA or RNA
• Easy to Use: Can be used as-is or diluted to the desired concentration

UltraPure™ Ethidium Bromide is an aromatic compound with a phenanthridine core (see structure below). Ethidium bromide has excitation maxima at 300 nm and 520 nm and an emission maximum at 600 nm (see fluorescence spectra).

For research use only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodFluorescence
Label or DyeEthidium bromide
Product TypeEthidium Bromide
Product LineUltraPure™
Quantity10 mL
Shipping ConditionRoom Temperature
Target MoleculeRNA, DNA
Unit SizeEach
Contents & Storage
10ml of 10mg⁄ml solution. Store at 15°C to 30°C

Frequently asked questions (FAQs)

Do you have a protocol for ethidium bromide staining in agarose gels?

For use in agarose gels:

- Add ethidium bromide to melted agarose to a final concentration of 0.5 µg/mL. Do not melt agarose that already contains ethidium bromide.

For staining agarose gels after electrophoresis:

- You can stain gels that have been run in the absence of ethidium bromide by covering the gel in 0.5 µg/mL ethidium bromide in water and gently agitating for 10 to 30 minutes.
- If necessary, gels can be destained by shaking in H2O for an additional 30 minutes. To stain RNA gels, you should minimize staining time and definitely include a destaining period.

What can ethidium bromide be used to detect and what is its sensitivity?

Ethidium bromide can be used to detect ssDNA, RNA, and dsDNA. This fluorescent dye intercalates between the stacked bases of nucleic acids, and exhibits an increased fluorescence at 590 nm. Ethidium bromide can detect down to ~1-10 ng/band of dsDNA in an agarose gel.