pMCS-Green Renilla Luc Vector for Luciferase Assays
pMCS-Green Renilla Luc Vector for Luciferase Assays
Thermo Scientific™

pMCS-Green Renilla Luc Vector for Luciferase Assays

The Thermo Scientific pMCS-Renilla Luc vector is a multiple cloning site plasmid to designed to accept a promoter sequence forRead more
Have Questions?
Catalog number 16152
Price (USD)
119.00
Each
Add to cart
Price (USD)
119.00
Each
Add to cart
The Thermo Scientific pMCS-Renilla Luc vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter.

Features of the pMCS-Renilla Luc vector:

• Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

The Green Renilla Luc Vectors contain a gene cloned from the sea pansy, Renilla reniformis. The gene encodes a bioluminescent Green Renilla luciferase (36kDa). The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.

These vectors are subject to a limited use label license.

More Product Data
Luciferase assays in hard-to-transfect Jurkat cells

Related Products
pCMV-Green Renilla Luc Vector for Luciferase Assays
pTK-Green Renilla Luc Vector for Luciferase Assays
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionpMCS-Green Renilla Luc Vector
TypeLuc Vector
Cloning MethodRestriction Enzyme/MCS
Delivery TypeTransfection
Quantity10 μg
Reporter GeneGreen Renilla Luciferase
Selection Agent (Eukaryotic)Puromycin
Product LinePierce™
For Use With (Application)Reporter Assays
Unit SizeEach
Contents & Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.

Frequently asked questions (FAQs)

What options do you offer for luciferase vectors?

There are three vectors available for each expressed luciferase; pMCS, pCMV, and pTK. We recommend using pMCS to measure the activity of the reporter, and pCMV, and pTK as positive control vectors. For high level constitutive expression of Luciferase, we recommend using the CMV (Cytomegalovirus) promoter vector and for low level constitutive expression of luciferase, we recommend using the pTK (thymidine kinase) promoter vector.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the stability of the secreted luciferase?

Gaussia, Gaussia-Dura, and Cypridina Luciferase are stable in the culture media for greater than 24 hours. Generally, after transfection of CMV driven constructs, we see ever increasing secreted signal between 24 and 72 hours.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How quickly is luciferase secreted?

The earliest detectable signal will depend on the strength of the promoter. In general, with a strong promoter (such as CMV), significant signal over background can be seen in as little as 20 minutes. However, with a weaker inducible promoter, significant signal over background may take 1-2 hours after induction.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you still offer quantitative IP (qIP) Luciferase assay kits?

Our qIP Luciferase assay kits have been discontinued but we do carry epitope-tagged (HA or c-Myc) and Tluc (TurboLuc luciferase)-tagged mammalian expression vectors, and qIP protein interaction assay reagents for these assay kits.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.